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Histone 3 抗体

H3 适用: Tomato, Arabidopsis thaliana, Zea mays, Moss, Chlamydomonas reinhardtii WB, ICC 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN619545
发货至: 中国
  • 抗原 See all Histone 3 (H3) 抗体
    Histone 3 (H3)
    适用
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    Tomato, Arabidopsis thaliana, Zea mays, Moss, Chlamydomonas reinhardtii
    宿主
    • 1183
    • 242
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    克隆类型
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    多克隆
    标记
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    This Histone 3 antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Immunocytochemistry (ICC)
    预测反应
    dicots including: Vitis vinifera, monocots: Hordeum vulgare, Oryza sativa, Zea mays, trees: Pinus pinaster, algae: Volvox sp.
    产品特性
    Expected / apparent Molecular Weight of the Antigene: 15 / 17 kDa
    纯化方法
    serum
    免疫原
    KLH-conjugated synthetic peptide derived from known H3 sequences, inluding Arabidopsis thaliana H3.3 P59169 (At4g40030,At4g40040,At4g10980), H3.2 P59226(At4g09200,At4g27360,At4g10390,At4g10400,At4g65360), H3-like 2 Q9FXI7 (At4g19890)
    Top Product
    Discover our top product H3 Primary Antibody
  • 应用备注
    Recommended Dilution: 1 : 5000 with standard ECL (WB), 1 : 100 - 1 : 500 (ICC)
    说明

    Protocol for isolation of cytosolic and nuclear fractions can be found here.Specific fluorescence in ICC has been observed for interphase nuclei as well as around centromer region (where Ser10 of histone H3 is phosphorylated) in mitotic chromosomes.

    限制
    仅限研究用
  • 状态
    Lyophilized
  • Sikorskaite, Rajamäki, Baniulis, Stanys, Valkonen: "Protocol: Optimised methodology for isolation of nuclei from leaves of species in the Solanaceae and Rosaceae families." in: Plant methods, Vol. 9, Issue 1, pp. 31, (2013) (PubMed).

    van der Linde, Hemetsberger, Kastner, Kaschani, van der Hoorn, Kumlehn, Doehlemann: "A maize cystatin suppresses host immunity by inhibiting apoplastic cysteine proteases." in: The Plant cell, Vol. 24, Issue 3, pp. 1285-300, (2012) (PubMed).

  • 抗原
    Histone 3 (H3)
    别名
    H3 (H3 产品)
    别名
    H-3 antibody, histocompatibility 3 antibody, H3 antibody
    背景
    AGI Code: At4g40030
    Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4.
    分子量
    expected: 15 kDa, apparent: 17 kDa
    UniProt
    P59169, P59226, Q9FXI7
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