MUC3A 抗体 (CF®405S)
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- 抗原 See all MUC3A products
- MUC3A (Mucin 3A, Cell Surface Associated (MUC3A))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This MUC3A antibody is conjugated to CF®405S
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应用范围
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 原理
- Mouse Monoclonal anti-Mucin 3 (M3.1), CF405S Conjugate
- 产品特性
- This antibody recognizes a protein of HMW, identified as mucin 3 glycoprotein (MUC3). Its epitope localizes between aa SITTTE. This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC2. MUC3 is distributed in colon and rectum, and is also present to a lesser extent in breast, lung and salivary gland tissues. The Mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (Mucin 1), 69 base pairs (Mucin 2) and 51 base pairs (Mucin 3). The number of repeats is highly polymorphic and varies among different alleles. Mucin 1 proteins are expressed as type I membrane proteins in addition to secreted forms. Mucin 1 is aberrantly expressed in epithelial tumors including breast carcinomas. Mucin 2 coats the epithelia of the intestines and airways and is associated with colonic tumors. Mucin 3 is a major component of various mucus gels and is broadly expressed in normal and tumor cells. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 免疫原
- A synthetic peptide of 35 amino acids, SIB35 (C-HSTPSFTSSITTTETTSHSTPSFTSSITTTETTS), which contains two of the MUC3 tandem repeats, coupled to KLH.
- 克隆位点
- M3-1
- 亚型
- IgG2a kappa
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anti-Mucin 3A, Cell Surface Associated (MUC3A) antibody
MUC3A 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal M3-1 unconjugated
anti-Mucin 3A, Cell Surface Associated (MUC3A) antibodyMUC3A 适用: 人 IF, FACS, IHC, StM 宿主: 小鼠 Monoclonal MUC3-1154 unconjugated
anti-Mucin 3A, Cell Surface Associated (MUC3A) (AA 1501-1600) antibodyMUC3A 适用: 人 IHC (p), ELISA, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mucin 3A, Cell Surface Associated (MUC3A) (AA 2176-2280) antibodyMUC3A 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mucin 3A, Cell Surface Associated (MUC3A) antibodyMUC3A 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Mucin 3A, Cell Surface Associated (MUC3A) antibodyMUC3A 适用: 人 IHC, StM 宿主: 兔 Monoclonal MUC3-2992R unconjugated Recombinant Antibody
anti-Mucin 3A, Cell Surface Associated (MUC3A) (AA 1-101) antibodyMUC3A 适用: 小鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mucin 3A, Cell Surface Associated (MUC3A) (AA 2236-2356) antibodyMUC3A 适用: 人 WB, IHC (p), ELISA, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Optimal dilution for a specific application should be determined by user
- 说明
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LS174T cells or colon carcinoma and normal colon tissue.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 100 μg/mL
- 缓冲液
- PBS/0.1 % BSA/0.05 % azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Protect from light
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- 抗原
- MUC3A (Mucin 3A, Cell Surface Associated (MUC3A))
- 别名
- Mucin 3 (MUC3A 产品)
- 别名
- Muc3 antibody, MUC-3A antibody, MUC3 antibody, mucin 3, intestinal antibody, mucin 3A, cell surface associated antibody, Muc3 antibody, MUC3A antibody
- 背景
- DOM, PCWH, SOX10, SRY (sex determining region Y) box 10, SRY box containing gene 10, SRY related HMG box gene 10, Waardenburg syndrome type 2E (WS2E), WS4, Waardenburg syndrome type 4C (WS4C)
- 分子量
- 1100 kDa
- 基因ID
- 4584, 57876
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