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- 抗原 See all MUC3A products
- MUC3A (Mucin 3A, Cell Surface Associated (MUC3A))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This MUC3A antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Staining Methods (StM)
- 特异性
- It recognizes a protein of HMW, identified as mucin 3 glycoprotein (MUC3). Its epitope localizes between aa SITTTE. This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC2. MUC3 is distributed in colon and rectum, and is also present to a lesser extent in breast, lung and salivary gland tissues. The Mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (Mucin 1), 69 base pairs (Mucin 2) and 51 base pairs (Mucin 3). The number of repeats is highly polymorphic and varies among different alleles. Mucin 1 proteins are expressed as type I membrane proteins in addition to secreted forms. Mucin 1 is aberrantly expressed in epithelial tumors including breast carcinomas. Mucin 2 coats the epithelia of the intestines and airways and is associated with colonic tumors. Mucin 3 is a major component of various mucus gels and is broadly expressed in normal and tumor cells.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- A synthetic peptide of 35 amino acids, SIB35 (C-HSTPSFTSSITTTETTSHSTPSFTSSITTTETTS), which contains two of the MUC3 tandem repeats, coupled to KLH.
- 克隆位点
- M3-1
- 亚型
- IgG2a kappa
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anti-Mucin 3A, Cell Surface Associated (MUC3A) antibody
MUC3A 适用: 人 IF, FACS, IHC, StM 宿主: 小鼠 Monoclonal MUC3-1154 unconjugated
anti-Mucin 3A, Cell Surface Associated (MUC3A) (AA 1501-1600) antibodyMUC3A 适用: 人 IHC (p), ELISA, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mucin 3A, Cell Surface Associated (MUC3A) (AA 2176-2280) antibodyMUC3A 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mucin 3A, Cell Surface Associated (MUC3A) antibodyMUC3A 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Mucin 3A, Cell Surface Associated (MUC3A) antibodyMUC3A 适用: 人 IHC, StM 宿主: 兔 Monoclonal MUC3-2992R unconjugated Recombinant Antibody
anti-Mucin 3A, Cell Surface Associated (MUC3A) (AA 1-101) antibodyMUC3A 适用: 小鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mucin 3A, Cell Surface Associated (MUC3A) (AA 2236-2356) antibodyMUC3A 适用: 人 WB, IHC (p), ELISA, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Positive Control: LS174T cells. Human small intestine (IHC).
Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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