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CD34 抗体 (CF®488A)

CD34 适用: 人 FACS, IF, IHC (f), ISt 宿主: 小鼠 Monoclonal QBEnd-10 HPCA1-763 CF®488A
产品编号 ABIN6185872
发货至: 中国
  • 抗原 See all CD34 抗体
    CD34
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    小鼠
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    This CD34 antibody is conjugated to CF®488A
    应用范围
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    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunostaining (ISt)
    原理
    Mouse Monoclonal anti-CD34 (QBEnd/10 + HPCA1/763), CF488A Conjugate
    产品特性
    This MAb recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120 kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, CD34 is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. Anti-CD34 labels > 85 % of angiosarcoma and Kaposi s sarcoma, but with a lower specificity. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    免疫原
    Detergent solubilized vesicular suspension prepared from human term placenta (QBEnd/10), Recombinant full-length human HPCA1 protein (HPCA1/763)
    克隆位点
    QBEnd-10 HPCA1-763
    亚型
    IgG
    Top Product
    Discover our top product CD34 Primary Antibody
  • 应用备注
    Immunohistology formalin-fixed 0.25-0.5 μg/mL
    • Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user
    说明

    KG-1 cells, Tonsil, or Angiosarcoma

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    100 μg/mL
    缓冲液
    PBS/0.1 % BSA/0.05 % azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Protect from light
  • 抗原
    CD34
    别名
    CD34 (CD34 产品)
    别名
    AU040960 antibody, CD34 molecule antibody, CD34 antigen antibody, CD34 antibody, Cd34 antibody
    背景
    Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin
    分子量
    90-110 kDa
    基因ID
    947, 374990
    UniProt
    P28906
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