ABO, Blood Group A Antigen 抗体
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- 抗原
- ABO, Blood Group A Antigen
- 适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Immunofluorescence (IF), Haemagglutination (H), Immunohistochemistry (IHC), Agglutination (AGG)
- 原理
- Mouse Monoclonal anti-Blood-Group-A (HE-10), Purified, with BSA
- 产品特性
- This MAb preferably reacts with determinants of chain A and H type 3(Gal1-3GalNAc-R) and 4 (Gal1-3GalNAc-R), but not with type 1 and 2 chain structures. It is not reactive with immuno-dominant A trisaccharide. This MAb is applicable for tissue staining in tumor patients with blood groups A and AB. It shows a highly heterogeneous reactivity in human colon tumor tissue and adjacent mucosa. Blood-group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 纯化方法
- Purified
- 免疫原
- Mixture of erythrocytes of blood group A and glycoprotein fraction isolated from the saliva of secretors with blood group A
- 克隆位点
- HE-10
- 亚型
- IgM kappa
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anti-ABO, Blood Group A Antigen antibody
适用: 人 IHC (p), AGG 宿主: 小鼠 Monoclonal HE-193 unconjugated
anti-ABO, Blood Group A Antigen antibody适用: 人 AGG 宿主: 小鼠 Monoclonal HE-195 unconjugated
anti-ABO, Blood Group A Antigen antibody适用: 人 FACS 宿主: 小鼠 Monoclonal HE-195 unconjugated
anti-ABO, Blood Group A Antigen antibody (PE)适用: 人 FACS 宿主: 小鼠 Monoclonal HE-193 PE
anti-ABO, Blood Group A Antigen antibody适用: 人 IHC (p), AGG 宿主: 小鼠 Monoclonal HE-193 unconjugated
anti-ABO, Blood Group A Antigen antibody适用: 人 IHC (p), IF 宿主: 小鼠 Monoclonal 3-3A unconjugated
anti-ABO, Blood Group A Antigen antibody适用: 人 IHC (p), IF, H 宿主: 小鼠 Monoclonal HE-193 unconjugated
anti-ABO, Blood Group A Antigen antibody适用: 人 IHC (p), IF, H 宿主: 小鼠 Monoclonal HE-193 unconjugated
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- 应用备注
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Agglutination
- Immunohistology (formalin)
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
- 说明
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KG1 cells or human colorectal carcinoma tissues.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 200 μg/mL
- 缓冲液
- PBS/0.05 % BSA/0.05 % azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- 抗原
- ABO, Blood Group A Antigen
- 别名
- Blood-Group-A
- 分子量
- Multiple
- 基因ID
- 28, 654423
- UniProt
- P16442
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