ABO, Blood Group A Antigen 抗体
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- 抗原
- ABO, Blood Group A Antigen
- 适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
- 产品特性
- This mAb preferably reacts with determinants of chain A and H type 3 (Gal1-3GalNAc-R) and 4 (Gal1-3GalNAc-R), but not with type 1 and 2 chain structures. It is not reactive with immuno-dominant A trisaccharide. This mAb is applicable for tissue staining in tumor patients with blood groups A and AB. It shows a highly heterogeneous reactivity in human colon tumor tissue and adjacent mucosa. Blood-group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Mucin isolated from an ovarian cyst fluid was used as the immunogen for the ABO antibody.
- 克隆位点
- 3-3A
- 亚型
- IgG1 kappa
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anti-ABO, Blood Group A Antigen antibody
适用: 人 IHC (p), AGG 宿主: 小鼠 Monoclonal HE-193 unconjugated
anti-ABO, Blood Group A Antigen antibody适用: 人 AGG 宿主: 小鼠 Monoclonal HE-195 unconjugated
anti-ABO, Blood Group A Antigen antibody适用: 人 FACS 宿主: 小鼠 Monoclonal HE-195 unconjugated
anti-ABO, Blood Group A Antigen antibody (PE)适用: 人 FACS 宿主: 小鼠 Monoclonal HE-193 PE
anti-ABO, Blood Group A Antigen antibody适用: 人 IHC (p), AGG 宿主: 小鼠 Monoclonal HE-193 unconjugated
anti-ABO, Blood Group A Antigen antibody适用: 人 IHC (p), IF, H 宿主: 小鼠 Monoclonal HE-193 unconjugated
anti-ABO, Blood Group A Antigen antibody适用: 人 IHC (p), IF, H 宿主: 小鼠 Monoclonal HE-193 unconjugated
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- 应用备注
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Optimal dilution of the ABO antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- 储存液
- Azide free
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the ABO antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- ABO, Blood Group A Antigen
- 别名
- ABO (Blood Group Antigen A)
- 背景
- This mAb preferably reacts with determinants of chain A and H type 3 (Gal1-3GalNAc-R) and 4 (Gal1-3GalNAc-R), but not with type 1 and 2 chain structures. It is not reactive with immuno-dominant A trisaccharide. This mAb is applicable for tissue staining in tumor patients with blood groups A and AB. It shows a highly heterogeneous reactivity in human colon tumor tissue and adjacent mucosa. Blood-group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
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