ICAM1 抗体 (CF®405S)
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- 抗原 See all ICAM1 抗体
- ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This ICAM1 antibody is conjugated to CF®405S
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 原理
- Mouse Monoclonal anti-CD54 (1H4-or-Wehi-CAM-1-or-W-CAM-1), CF405S Conjugate
- 产品特性
- This antibody recognizes an 85-115 kDa protein (variation with cell type), identified as intercellular adhesion molecule (ICAM-1) (Workshop IV). It has 7 potential N-linked glycosylation sites. ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1). This interaction enhances antigen-specific T-cell activation. ICAM-1 also binds to CD43 and to Plasmodium falciparum infected RBCs. W-CAM-1 MAb blocks aggregation of cell lines mediated by the ICAM-1 and blocks homotypic binding of purified populations of activated T- and B-lymphocytes and also aggregation of mixed T- and B-cell blasts. It inhibits T-cell adhesion to normal human endothelial cells. Activation induced by cell-cell contact (mixed lymphocyte reaction, T-cell mediated B-cell activation) is significantly inhibited. This MAb blocks elements of both effector arms of immune system (cytotoxic cell function and Ig production). Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 免疫原
- Raji Burkitt lymphoma cells
- 克隆位点
- 1H4 or Wehi-CAM-1 or W-CAM-1
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product ICAM1 Primary Antibody
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anti-Intercellular Adhesion Molecule 1 (ICAM1) (AA 201-300) antibody
ICAM1 适用: 人, 小鼠, 大鼠, 兔 WB, FACS, ELISA, IHC (p), ICC, IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Intercellular Adhesion Molecule 1 (ICAM1) (AA 198-537) antibodyICAM1 适用: 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Intercellular Adhesion Molecule 1 (ICAM1) antibodyICAM1 适用: 人, 大鼠, Cow WB, FACS, ELISA, IHC (p), ICC 宿主: 小鼠 Monoclonal MEM-111 unconjugated
anti-Intercellular Adhesion Molecule 1 (ICAM1) (AA 102-306) antibodyICAM1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Intercellular Adhesion Molecule 1 (ICAM1) (AA 41-464) antibodyICAM1 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C2 unconjugated
anti-Intercellular Adhesion Molecule 1 (ICAM1) (pTyr512) antibodyICAM1 适用: 人, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Intercellular Adhesion Molecule 1 (ICAM1) (AA 41-464) antibodyICAM1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Intercellular Adhesion Molecule 1 (ICAM1) (AA 27-209) antibodyICAM1 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Intercellular Adhesion Molecule 1 (ICAM1) (AA 1-532) antibodyICAM1 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 3H8-2G6 unconjugated
anti-Intercellular Adhesion Molecule 1 (ICAM1) (AA 313-327) antibodyVerified ICAM1 适用: 人 WB, FACS, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
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- 应用备注
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Immunohistology formalin-fixed 2-4 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Functional Studies order Ab without BSA & azide)
- Optimal dilution for a specific application should be determined by user
- 说明
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Raji cells, MOLT 4, HeLa or SW480 cells. Human tonsil.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 100 μg/mL
- 缓冲液
- PBS/0.1 % BSA/0.05 % azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Protect from light
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- 抗原
- ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))
- 别名
- CD54 (ICAM1 产品)
- 别名
- BB2 antibody, CD54 antibody, P3.58 antibody, Icam-1 antibody, Ly-47 antibody, MALA-2 antibody, ICAM antibody, ICAM-1 antibody, ICAM1 antibody, intercellular adhesion molecule 1 antibody, intercellular adhesion molecule-1 antibody, ICAM1 antibody, Icam1 antibody, ICAM-1 antibody
- 物质类
- Viral Protein
- 背景
- Intestinal mucin-3A, intestinal mucin-like, MUC3, MUC3A, MUC3B, mucin 3, intestinal, mucin 3A, cell surface associated, mucin 3A, intestinal, mucin 3B, cell surface associated
- 分子量
- 85-115 kDa
- 基因ID
- 3383, 643447
- UniProt
- P05362
- 途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling
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