Mouse Monoclonal anti-CD30 (CD30/412), Purified, with BSA
产品特性
This antibody recognizes a single chain glycoprotein of 105/120 kDa, identified as CD30/Ki-1. CD30 is synthesized as a 90 kDa precursor, which is processed in the Golgi complex into a membrane-bound phosphorylated mature 105/120 kDa glycoprotein. In Hodgkin's disease, CD30/Ki-1 antigen is expressed by mononuclear-Hodgkin and multinucleated Reed-Sternberg cells. It is also expressed by the tumor cells of a majority of anaplastic large cell lymphomas as well as by a varying proportion of activated T and B cells. This MAb distinguishes large cell lymphomas derived from activated lymphoid cells from histiocytic malignancies and lymphomas derived from resting and precursor lymphoid cells or from anaplastic carcinomas. About one third of the Ki-1 positive lymphomas lack the leukocyte common antigen (CD45). Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
TNFRSF8
适用: 大鼠
ELISA, IHC (p), IF (p), IF (cc), ICC, IHC (fro)
宿主: 兔
Polyclonal
unconjugated
应用备注
Immunohistology formalin-fixed 0.5-1 μg/mL
Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
Flow Cytometry 0.5-1 μg/million cells/0.1 mL
Immunofluorescence 1-2 μg/mL
Optimal dilution for a specific application should be determined by user
限制
仅限研究用
状态
Liquid
浓度
200 μg/mL
缓冲液
PBS/0.05 % BSA/0.05 % azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
抗原
TNFRSF8
(Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))