TNFRSF8 抗体
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- 抗原 See all TNFRSF8 抗体
- TNFRSF8 (Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This TNFRSF8 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
- 特异性
- This antibody reacts with CD30 antigen (120 kDa) on Western blotting, Immunoprecipitation, and Flow Cytometry.
- 交叉反应 （详细）
- Species reactivity (tested):Human.
- 产品特性
- Synonyms: TNFRSF8, D1S166E, CD30L receptor, KI-1 antigen, Tumor necrosis factor receptorsuperfamily member 8, Lymphocyte activation antigen CD30
- 纯化方法
- Protein-A Agarose Chromatography of hybridoma supernatant.
- 免疫原
- Co cell line cellsRemarks: Hybridoma was established by fusion of mouse myeloma cell with Balb/c mousesplenocyte
- 克隆位点
- Ber-H2
- 亚型
- IgG1
- Top Product
- Discover our top product TNFRSF8 Primary Antibody
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anti-Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) (AA 19-379) antibody
TNFRSF8 适用: 人 IHC, WB, IHC (p) 宿主: 小鼠 Monoclonal 18D3 unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) antibodyTNFRSF8 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 3B10 unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) antibodyTNFRSF8 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) (AA 19-379) antibodyTNFRSF8 适用: 人 FACS, IHC, WB, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 4D1C2 unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) (AA 131-230) antibodyTNFRSF8 适用: 人, Pig FACS, WB, IHC (p), ELISA, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) antibodyTNFRSF8 适用: 人 FACS, IHC (p) 宿主: 小鼠 Monoclonal MEM-268 unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) antibody (APC)TNFRSF8 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-268 APC
anti-Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) (AA 51-150) antibodyTNFRSF8 适用: 大鼠 IHC (p), ELISA, IF (p), IF (cc), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) antibodyTNFRSF8 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) antibodyTNFRSF8 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal rCD30-412 unconjugated Recombinant Antibody
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- 应用备注
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Western blotting: 1 μg/mL for chemiluminescence detection system. Positive Control: CCRF-CEM. Immunoprecipitation: 2 μg/200 μL of cell extract from 5x10^6 cells. Flow Cytometry: 10 μg/mL (final concentration). Immunohistochemistry on Paraffin Embedded Section: 1-5 μg/mL (Heat treatment isnecessary). Microwave oven, 2 times for 10 minutes each in 10 mM Citrate buffer ( pH 6.5). Detailed procedure is provided in Protocols.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- SDS-PAGE & Western Blotting1) Wash the cells 3 times with PBS and suspend with 10 volume of cold Lysis buffer (50 mMTris-HCl, pH 7. 2, 250 mM NaCl, 0. 1% NP-40, 2 mM EDTA, 10%glycerol) containingappropriate protease inhibitors. Incubate it at 4°C with rotating for 30 minutes, thensonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4°C and transfer the supernatant toanother tube. Measure the protein concentration of the supernatant and add the cold Lysisbuffer to make 8 mg/mL solution. 3) Mix the sample with equal volume of Laemmli's sample buffer. 4) Boil the samples for 3 minutes and centrifuge. Load 10 μL of the sample per lane in a 1mm thick SDS-polyacrylamide gel for electrophoresis. 5) Blot the protein to a polyvinylidene difluoride (PVDF) membrane at 1 mA/cm2 for 1 hourin a semi-dry transfer system (Transfer Buffer: 25 mM Tris, 190 mM glycine, 20% MeOH). See the manufacture's manual for precise transfer procedure. 6) To reduce nonspecific binding, soak the membrane in 10% skimmed milk (in PBS, pH7. 2) for 1 hour at room temperature, or overnight at 4°C. 7) Incubate the membrane with primary antibody diluted with PBS, pH 7. 2 containing 1%skimmed milk as suggest in the APPLICATIONS for 1 hour at room temperature. (Theconcentration of antibody will depend on condition. )8) Wash the membrane with PBS-T [0. 05% Tween-20 in PBS] (5 minutes x 3 times). 9) Incubate the membrane with the 1: 10,000 HRP-conjugated anti-mouse IgG diluted with1% skimmed milk (in PBS, pH 7. 2) for 1 hour at RT. 10) Wash the membrane with PBS-T (10 minutes x 3 times). 11) Wipe excess buffer on the membrane, then incubate it with appropriatechemiluminescence reagent for 1 minute. 12) Remove extra reagent from the membrane by dabbing with paper towel, and seal it inplastic wrap. 13) Expose to an X-ray film in a dark room for 3 minutes. 14) Develop the film as usual. The condition for exposure and development may vary. Positive Control: U251, CCRF-CEM. Immunoprecipitation1) Wash the cells 3 x with PBS and suspend with 10 volume of cold Lysis buffer (50 mMTris-HCl pH 7. 2, 250 mM NaCl, 0. 1% NP-40, 2 mM EDTA, 10% glycerol) containingappropriate protease inhibitors. Incubate it at 4°C with rotating for 30 minutes, thensonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4°C and transfer the supernatant toanother tube.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH 7.2 containing 50 % Glycerol without preservatives.
- 储存液
- Without preservative
- 储存条件
- -20 °C
- 储存方法
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Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- TNFRSF8 (Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))
- 别名
- CD30 (TNFRSF8 产品)
- 别名
- CD30 antibody, D1S166E antibody, Ki-1 antibody, Cd30 antibody, Ki antibody, TNFRSF8 antibody, TNF receptor superfamily member 8 antibody, tumor necrosis factor receptor superfamily, member 8 antibody, TNFRSF8 antibody, Tnfrsf8 antibody
- 背景
- CD30, also known as Ki-1, TNFRSF8, or Be-H2, is a 120 kDa glycoprotein expressed on the surface of mitogen-activated B-cells and T-cells but not on resting lymphocytes or monocytes. CD30 is also a marker for Hodgkin and Sternberg-Reed cells of Hodgkin's lymphomas and related hematologic malignancies. Soluble forms of CD30 have been found in the serum of patients with adult T-cell leukemia or other CD30+ lymphomas. The CD30 ligand, CD153, is a type II transmembrane glycoprotein that enhances proliferation of activated T-cells and induces apoptosis in CD30+ lymphoma-derived cell lines.Synonyms: CD30L receptor, D1S166E, KI-1 antigen, Lymphocyte activation antigen CD30, TNFRSF8, Tumor necrosis factor receptor superfamily member 8
- 基因ID
- 943
- UniProt
- P28908
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