CD99 抗体
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- 抗原 See all CD99 抗体
- CD99
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD99 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 原理
- Mouse Monoclonal anti-CD99 (MIC2/877), Purified, BSA-free
- 产品特性
- This antibody recognizes a sialoglycoprotein of 27-32 kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32 kDa (p30/32).Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis.CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing s sarcoma cells and primitive peripheral neuroectodermal tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 纯化方法
- Purified
- 免疫原
- Recombinant full-length human MIC2 protein
- 克隆位点
- MIC2-877
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product CD99 Primary Antibody
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anti-CD99 (CD99) (AA 1-185) antibody
CD99 适用: 人 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 1B10E12 unconjugated
anti-CD99 (CD99) (AA 1-185) antibodyCD99 适用: 人 FACS, WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 7E11C4 unconjugated
anti-CD99 (CD99) (AA 23-122) antibodyCD99 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3A10 unconjugated
anti-CD99 (CD99) (AA 23-185) antibodyCD99 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD99 (CD99) (AA 27-161) antibodyCD99 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD99 (CD99) (AA 26-160) antibodyCD99 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD99 (CD99) antibodyCD99 适用: 人 FACS, CyTOF 宿主: 小鼠 Monoclonal 3B2-TA8 unconjugated
anti-CD99 (CD99) antibodyCD99 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 5C8 unconjugated
anti-CD99 (CD99) antibody (PE)CD99 适用: 人 FACS 宿主: 小鼠 Monoclonal 3B2-TA8 PE
anti-CD99 (CD99) antibodyCD99 适用: 人 FACS, WB, IHC, ELISA 宿主: 兔 Monoclonal 9A5 unconjugated Recombinant Antibody
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- 应用备注
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Immunohistology formalin-fixed 1-2 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Does not react with rat, others not tested
- Optimal dilution for a specific application should be determined by user
- 说明
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MOLT-4 cells. Pancreas or Ewing s sarcoma
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS (no BSA, no azide)
- 储存液
- Azide free
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- 抗原
- CD99
- 别名
- CD99 (CD99 产品)
- 别名
- 1110061M03Rik antibody, 2410026K10Rik antibody, D4 antibody, Pilr-l antibody, pilr-1 antibody, HBA71 antibody, MIC2 antibody, MIC2X antibody, MIC2Y antibody, MSK5X antibody, CD99 antigen antibody, CD99 molecule (Xg blood group) antibody, CD99 molecule antibody, Cd99 antibody, CD99 antibody
- 物质类
- Viral Protein
- 背景
- 12E7, E2 antigen, MIC 2X, MIC 2Y, MIC2, Protein MIC2, Surface antigen MIC2, T-cell surface glycoprotein E2
- 分子量
- 27-32 kDa
- 基因ID
- 4267, 653349
- UniProt
- P14209
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