Insulin 抗体
Quick Overview for Insulin 抗体 (ABIN6157480)
抗原
See all Insulin (INS) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse Monoclonal anti-Insulin (IRDN/794), Purified, BSA-free
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产品特性
- This antibody recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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纯化方法
- Purified
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免疫原
- Recombinant INS protein
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亚型
- IgG1 kappa
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anti-Insulin (INS) (AA 1-110) antibody
INS 适用: 人 IHC, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Insulin (INS) (AA 46-59) antibodyINS 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), FACS, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Insulin (INS) antibodyINS 适用: 人 IHC, WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Insulin (INS) (AA 90-110) antibodyINS 适用: 小鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Insulin (INS) (AA 25-110) antibodyINS 适用: 大鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Insulin (INS) antibodyINS 适用: 人, Pig, 小鼠, Cow, 大鼠, 兔, 豚鼠, 犬, 猴, Avian, 绵羊, 马, Hamster, 山羊, 驴, 小鸡, Cat WB 宿主: 山羊 Polyclonal unconjugated
anti-Insulin (INS) (AA 25-110) antibodyINS 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Insulin (INS) (AA 25-110) antibodyINS 适用: 人 IHC, WB, IP, ICC 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Insulin (INS) (AA 25-54) antibodyINS 适用: Cow IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Insulin (INS) antibodyINS 适用: 人 IHC, ELISA 宿主: 兔 Monoclonal 1F6 unconjugated Recombinant Antibody
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应用备注
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Immunohistology formalin-fixed 0.1-0.2 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Optimal dilution for a specific application should be determined by user
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说明
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MIA PaCa-2 cells or Pancreas
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS (no BSA, no azide)
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储存液
- Azide free
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- Insulin (INS)
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别名
- Insulin
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背景
- IDDM2, ILPR, IRDN, MODY10, Proinsulin
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分子量
- 6 kDa
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基因ID
- 3630, 272259
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UniProt
- P01308
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途径
- NF-kappaB Signaling, RTK signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
抗原
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