Histone H1 抗体 (CF®568)
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- 抗原 See all Histone H1 抗体
- Histone H1
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适用
- 人, 小鼠, 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Histone H1 antibody is conjugated to CF®568
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应用范围
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 原理
- Mouse Monoclonal anti-Histone H1 (AE-4), CF568 Conjugate
- 产品特性
- Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer, formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80 % of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 免疫原
- Nuclei of human leukemia biopsy cells
- 克隆位点
- AE-4
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product Histone H1 Primary Antibody
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anti-Histone H1 antibody
适用: 人, 小鼠, 大鼠 IF, FACS, IHC, StM 宿主: 小鼠 Monoclonal r1415-1 unconjugated Recombinant Antibody
anti-Histone H1 antibody适用: 人, 小鼠, 大鼠 IF, FACS, IHC, StM, CUT&RUN 宿主: 小鼠 Monoclonal AE-4 unconjugated
anti-Histone H1 (AA 1-105) antibody适用: 人 IF, IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 antibody适用: 人, 小鼠, 大鼠 IF, FACS, IHC, StM 宿主: 兔 Monoclonal HH1-1784R unconjugated Recombinant Antibody
anti-Histone H1 (acLys52) antibody适用: 人, 小鼠, 大鼠 FACS, IHC (p), ELISA, IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 antibody适用: 人, 小鼠, 大鼠 IF, FACS, IHC, StM 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 (AA 150-207) antibody适用: 人 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 (Lys1535) antibody适用: 人, 小鼠 IF, IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 (AA 1-194) antibody适用: 人 IF, IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 antibody适用: 人 IHC, WB 宿主: 兔 Monoclonal unconjugated
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- 应用备注
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- 说明
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HeLa, A-431, LNCap or Jurkat cells. Breast carcinoma.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 100 μg/mL
- 缓冲液
- PBS/0.1 % BSA/0.05 % azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Protect from light
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- 抗原
- Histone H1
- Abstract
- Histone H1 产品
- 别名
- H10 antibody, H1FV antibody, H1 histone family member 0 antibody, H1F0 antibody
- 分子量
- ~30 kDa
- 基因ID
- 3005
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