TRIM29 抗体 (Internal Region)
Quick Overview for TRIM29 抗体 (Internal Region) (ABIN571104)
抗原
See all TRIM29 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- TRIM29 / ATDC
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序列
- EKDRIKSFTT NE
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交叉反应
- Cow, 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-EKDRIKSFTTNE, from the internal region of the protein sequence according to NP_036233.2.
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亚型
- IgG
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应用备注
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Immunohistochemistry: In paraffin embedded Human Skin shows staining of subnuclear structures and cytoplasm in keratinocytes. Recommended concentration: 3.75 μg/mL.
Western Blot: Approx 37 kDa band observed in Human Peripheral Blood Mononucleocytes (PBM) and in lysates of cell lines HeLa. An additional 70 kDa band was seen in some PBM lysates (calculated MW of 37.3 kDa according to NP_001317311.1 (isoform 2) and 65.8
Peptide ELISA: antibody detection limit dilution 1:128000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the Cytoplasm and Intermediate filaments of A431 cells. Strong expression of the protein seen in the Nucleus of U2OS cells. Recommended concentration: 10μg/ml.
Flow C
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- TRIM29 (Tripartite Motif Containing 29 (TRIM29))
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别名
- TRIM29
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背景
- TRIM29, tripartite motif-containing 29, ATDC, FLJ36085, ataxia-telangiectasia group D-associated protein, tripartite motif protein TRIM29
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分子量
- 65.8kDa
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基因ID
- 23650, 72169, 300656
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NCBI登录号
- NP_036233, NP_001317311
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途径
- p53 Pathway, Apoptosis, DNA Damage Repair, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus
抗原
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