MEK1 抗体 (C-Term) (HRP)
Quick Overview for MEK1 抗体 (C-Term) (HRP) (ABIN5706851)
抗原
See all MEK1 (MAP2K1) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- C-Term
-
Supplier Product No.
- 600-403-gq0
-
Supplier
- Rockland
-
原理
- MEK1 C-Term Antibody HRP
-
交叉反应 (详细)
- This HRP conjugated affinity purified antibody is directed against human MEK1 protein.
-
产品特性
- Synonyms: rabbit anti-MEK1 antibody peroxidase conjugation, HRP conjugated rabbit anti-MEK1 antibody, Dual specificity mitogen-activated protein kinase kinase 1, MAP kinase kinase 1, MAPKK 1, MAP2K1, MEK, MEK 1, MKK1, PRKMK1, MEK-1, ERK activator kinase 1, MAPK/ERK kinase 1
-
纯化方法
- Anti-MEK1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using synthetic peptide coupled to agarose beads followed by cross adsorption to remove any unwanted reactivity.
-
免疫原
-
Immunogen: Anti-MEK1 Antibody was produced in rabbits by repeated immunizations with synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH.
Immunogen Type: Conjugated Peptide
-
亚型
- IgG
-
-
-
-
应用备注
-
Application Note: Anti-MEK 1 Peroxidase conjugated antibody has been tested by ELISA and is suitable for use in Western Blotting. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 43.5 kDa.
Western Blot Dilution: 1 μg/mL
FLISA Dilution: 1:40,000
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
-
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
-
浓度
- 1.0 mg/mL
-
缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
-
注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
- MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))
-
别名
- MAP2K1
-
背景
- Background: MEK1 antibodies detect the MEK1 isoform. Mitogen-activated protein kinase kinase 1, also known as MKK or MEK, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain. Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. The MEK1 antibody is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.
-
UniProt
- Q02750
-
途径
- MAPK Pathway, RTK signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling
抗原
-