Histone 3 抗体 (H3K9me2, pThr6)
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Quick Overview for Histone 3 抗体 (H3K9me2, pThr6) (ABIN5706786)
抗原
See all Histone 3 (H3) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- H3K9me2, pThr6
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Supplier Product No.
- 600-401-i65
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Supplier
- Rockland
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原理
- Histone H3 K9me2/phospho T6 Antibody
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交叉反应 （详细）
- This antibody reacts with human Histone H3.
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产品特性
- Synonyms: rabbit anti-Histone H3 pT6 dimethyl Lys9 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3K9me2/pT6
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纯化方法
- Anti-Histone H3 [p Thr6, Dimethyl Lys9] was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Histone H3 [p Thr6, Dimethyl Lys9] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic dimethylated/phosphorylated peptide surrounding Lysine 9/Threonine 6 of human Histone H3.2.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Histone H3 (H3) (H3K4me) antibody
H3 适用: 人 WB, IF, ChIP, IP, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (3meLys4) antibodyH3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3K27ac) antibodyH3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3K9ac) antibodyH3 适用: 人, 小鼠 WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3K36me3) antibodyH3 适用: 人, 小鼠 WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 小鼠 Monoclonal MABI 0333 unconjugated
anti-Histone H3 (H3) (AA 71-136) antibodyH3 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3K27me) antibodyH3 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3K4me3) antibodyH3 适用: 人, 小鼠, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (C-Term) antibodyH3 适用: 人 WB, IHC, ChIP, IP 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3R2me2) antibodyH3 适用: 人 WB, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: 1:100
Application Note: Anti-Histone H3 [p Thr6, Dimethyl Lys9] antibody is tested for Western Blot, Immunofluorescence, and ChIP. This antibody is suitable for Immunocytochemistry and Chromatin Immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.
ChIP Dilution: 2-5 μg/million cells
Western Blot Dilution: 1:500
IF Microscopy Dilution: 1:100
Other: Dot Blot 1:1000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.49 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Histone 3 (H3) (Histone H3 (H3))
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别名
- Histone H3
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背景
- Background: The doubly modified dimethyl K9, phospho-T6 histone H3 is known to exist, but very little comprehensive information about the importance of this modification and its mechanism has been published. H3K9me2 is a histone post-translational modification that is enriched in promoters of transcriptionally active genes. It is also important in cellular differentiation and maturation. Phosphorylation of histone H3 is also associated with mitosis and meiosis, and seems to be related to developmental changes. The dual modification of H3K9me2/pT6 is still under investigation and will present more insight into the complex epigenetic code. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.
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基因ID
- 126961
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NCBI登录号
- NP_001005464
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UniProt
- Q71DI3
抗原
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