Histone 3 抗体 (H3K18me2)
-
- 抗原 See all Histone 3 (H3) 抗体
- Histone 3 (H3)
-
抗原表位
- H3K18me2
-
适用
- 人, 小鼠, C. elegans
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This Histone 3 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Multiplex Assay (MA), Fluorescence Microscopy (FM)
- Supplier Product No.
- 600-401-i79
- Supplier
- Rockland
- 原理
- Histone H3 K18me2 Antibody
- 交叉反应 （详细）
- This antibody reacts with human Histone H3.
- 产品特性
- Synonyms: rabbit anti-Histone H3 dimethyl Lys18 antibody, H3.3AH3F3H3F3B, H3.3B, H3 histone, family 3A, histone H3.3, MGC87783, MGC87782, H3K18me2
- 纯化方法
- Anti-Histone H3 [Dimethyl Lys18] was affinity purified from monospecific antiserum by immunoaffinity chromatography.
- 过滤
- Sterile filtered
- 免疫原
-
Immunogen: Histone H3 [Dimethyl Lys18] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic dimethylated peptide surrounding Lysine 18 of human Histone H3.2.
Immunogen Type: Conjugated Peptide
- 亚型
- IgG
- Top Product
- Discover our top product H3 Primary Antibody
-
anti-Histone 3 (H3) (H3K4me) antibody
H3 适用: 人 WB, IF, ChIP, IP, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (H3K27ac) antibodyH3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (3meLys4) antibodyH3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (H3K36me3) antibodyH3 适用: 人, 小鼠 WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 小鼠 Monoclonal MABI 0333 unconjugated
anti-Histone 3 (H3) (H3K9ac) antibodyH3 适用: 人, 小鼠 WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (AA 71-136) antibodyH3 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, IHC (p), IF (cc), FACS, IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (H3K27me) antibodyH3 适用: 人 WB, IF, IHC, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (H3K4me3) antibodyH3 适用: 人, 小鼠, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (H3K36me3) antibodyH3 适用: 人 WB, IF, IHC, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (H3K36me2) antibodyH3 适用: 人 WB, IF, IHC, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
-
Immunohistochemistry Dilution: 1:50
Application Note: Anti-Histone H3 [Dimethyl Lys18] antibody is tested in Western Blot, Chromatin Immunoprecipitation, Dot Blot, and Immunofluorescence. This antibody is useful for Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.
ChIP Dilution: 2-5 μg/million cells
Western Blot Dilution: 1:500
IF Microscopy Dilution: 1:50
Other: Dot Blot 1:1000
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.79 mg/mL
- 缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 30 % Glycerol
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
-
- 抗原
- Histone 3 (H3)
- 别名
- Histone H3 (H3 产品)
- 背景
- Background: The di-methylated K18 on histone H3 is a seemingly transient post-translational modification. H3K18 is better known to be acetylated, and occasionally mono-methylated. Suv39h1, a well-studied histone methyltransferase seems to be responsible for the transition of acetylation and methylation at this H3 modification site. The di-methylated K18 on H3 seems to be associated with embryological development and possibly implantation. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.
- 基因ID
- 126961
- NCBI登录号
- NP_001005464
- UniProt
- Q71DI3
-
