BAFF antibody can be used to recognize membrane-bound human BAFF by immunocytochemistry and western blot. Anti-BAFF (MAb Buffy-1) detects endogenous protein by Flow Cytometry. Method: 293T cells (5 x 105) expressing surface BAFF vector or Mock transfected, were incubated on ice for 30 min in 50 μ,l FACS buffer (PBS, 5 % fetal calf serum, 0.02 % azide) containing 4 μ,g/mL of Buffy-1 antibody. After washing in FACS buffer, FITC-conjugated antibody to rat IgG was added and cells were analyzed by flow cytometry.
限制
仅限研究用
状态
Liquid
缓冲液
PBS containing 0.02 % sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C
储存方法
BAFF antibody can be stored at 4°C, stable for one year.
抗原
BAFF (TNFSF13B)
(Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))