ELISA: (direct and indirect: 1:2,000-1:5,000). Western Blot: (1:2,000-1:5,000 using ECL, suggested blocking and dilution buffer is PBST containing 0.05 % Tween 20 and 5 % skim milk, suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
储存液
Without preservative
储存条件
-20 °C
储存方法
Stable for at least 6 months after receipt when stored at -20°C.
AA589638 antibody, AI265570 antibody, Scd antibody, Scd-1 antibody, ab antibody, stearoyl-Coenzyme A desaturase 1 antibody, Scd1 antibody
背景
Stearoyl-CoA desaturase (SCD) is a regulatory enzyme in lipogenesis. The enzyme is constitutively expressed in adipose tissue and is induced in liver in response to fat free, high carbohydrate diet. Several SCD isoforms (SCD1, -2, and -3) exist in the mouse. Mice with a targeted disruption of the SCD1 isoform had reduced body adiposity, increased insulin sensitivity, and resistance to diet-induced weight gain. The protection from obesity involved increased energy expenditure and increased oxygen consumption. SCD1 deficiency shows activation of lipid oxidation in addition to reduced triglyceride synthesis and storage.