TNFSF14 抗体 (Biotin)
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- 抗原 See all TNFSF14 抗体
- TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This TNFSF14 antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA
- 纯化方法
- Anti-hLIGHT specific antibody was purified by affinity chromatography and then biotinylated.
- 免疫原
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hLIGHT (human LIGHT).
- Top Product
- Discover our top product TNFSF14 Primary Antibody
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- 应用备注
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ELISA:
To detect hLIGHT by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15-0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hLIGHT.
Sandwich:
To detect hLIGHT by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human LIGHT (XP-5215) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hLIGHT.
Western Blot:
To detect hLIGHT by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLIGHT is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 储存条件
- -20 °C
- 储存方法
- LIGHT antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- 抗原
- TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))
- 别名
- LIGHT (TNFSF14 产品)
- 别名
- TNFSF14 antibody, CD258 antibody, HVEML antibody, LIGHT antibody, LTg antibody, TR2 antibody, HVEM-L antibody, Ly113 antibody, TNF superfamily member 14 antibody, tumor necrosis factor (ligand) superfamily, member 14 antibody, TNFSF14 antibody, Tnfsf14 antibody
- 背景
- LIGHT is a type II membrane protein belonging to the TNF family of membrane-anchored ligands. It is expressed in splenocytes, activated PBL, CD8+ tumor infiltrating lymphocytes, granulocytes and monocytes. LIGHT has the ability to activate NFkB, stimulate proliferation of lymphocytes, and induce apoptosis in certain human tumor cells.
- 基因ID
- 8740
- UniProt
- O43557
- 途径
- Cancer Immune Checkpoints
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