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TNFSF14 抗体 (Biotin)

TNFSF14 适用: 人 WB, ELISA 宿主: 兔 Polyclonal Biotin
产品编号 ABIN5689060
发货至: 中国
  • 抗原 See all TNFSF14 抗体
    TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))
    适用
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    克隆类型
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    多克隆
    标记
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    This TNFSF14 antibody is conjugated to Biotin
    应用范围
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    Western Blotting (WB), ELISA
    纯化方法
    Anti-hLIGHT specific antibody was purified by affinity chromatography and then biotinylated.
    免疫原
    Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hLIGHT (human LIGHT).
    Top Product
    Discover our top product TNFSF14 Primary Antibody
  • 应用备注
    ELISA:

    To detect hLIGHT by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15-0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hLIGHT.

    Sandwich:

    To detect hLIGHT by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human LIGHT (XP-5215) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hLIGHT.

    Western Blot:

    To detect hLIGHT by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLIGHT is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
    限制
    仅限研究用
  • 状态
    Lyophilized
    储存条件
    -20 °C
    储存方法
    LIGHT antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
  • 抗原
    TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))
    别名
    LIGHT (TNFSF14 产品)
    别名
    TNFSF14 antibody, CD258 antibody, HVEML antibody, LIGHT antibody, LTg antibody, TR2 antibody, HVEM-L antibody, Ly113 antibody, TNF superfamily member 14 antibody, tumor necrosis factor (ligand) superfamily, member 14 antibody, TNFSF14 antibody, Tnfsf14 antibody
    背景
    LIGHT is a type II membrane protein belonging to the TNF family of membrane-anchored ligands. It is expressed in splenocytes, activated PBL, CD8+ tumor infiltrating lymphocytes, granulocytes and monocytes. LIGHT has the ability to activate NFkB, stimulate proliferation of lymphocytes, and induce apoptosis in certain human tumor cells.
    基因ID
    8740
    UniProt
    O43557
    途径
    Cancer Immune Checkpoints
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