电话:
400-7060-959
传真:
+86 10 56315212-8813
电子邮件:
orders@antibodies-online.cn

IL-3 抗体

IL-3 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN5689043
发货至: 中国
  • 抗原 See all IL-3 抗体
    IL-3 (Interleukin 3 (IL-3))
    适用
    • 72
    • 35
    • 23
    • 4
    宿主
    • 78
    • 17
    • 17
    • 2
    • 2
    克隆类型
    • 84
    • 32
    多克隆
    标记
    • 61
    • 17
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This IL-3 antibody is un-conjugated
    应用范围
    • 88
    • 46
    • 26
    • 26
    • 20
    • 15
    • 10
    • 10
    • 10
    • 9
    • 9
    • 8
    • 6
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Neutralization (Neut)
    纯化方法
    Anti-hIL-3 specific antibody was purified by affinity chromatography employing immobilized hIL-3 matrix.
    免疫原
    Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-3 (human Interleukin-3).
    Top Product
    Discover our top product IL-3 Primary Antibody
  • 应用备注
    Neutralization:
    To yield one-half maximal inhibition [ND50] of the biological activity of hIL-3 (1.0 ng/mL), a concentration of 0.38 - 0.57 μ,g/mL of this antibody is required.

    ELISA:

    To detect hIL-3 by indirect ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-3.

    Sandwich

    To detect hIL-3 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-3 (XP-5185Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-3.

    Western Blot:

    To detect hIL-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
    限制
    仅限研究用
  • 状态
    Lyophilized
    储存条件
    -20 °C
    储存方法
    IL-3 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
  • 抗原
    IL-3 (Interleukin 3 (IL-3))
    别名
    IL-3 (IL-3 产品)
    别名
    BPA antibody, Csfmu antibody, HCGF antibody, Il-3 antibody, MCGF antibody, PSF antibody, IL-3 antibody, MULTI-CSF antibody, interleukin 3 antibody, interleukin 3 (colony-stimulating factor, multiple) antibody, Il3 antibody, IL3 antibody
    背景
    The pleiotropic IL-3 (Interleukin 3) is a 15  kDa cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL-3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells. IL-3 not only supports growth of both pluripotent stem cells and the more differentiated committed progenitors, but it also stimulates the functional activity of some fully differentiated cells. IL-3 has also been shown to protect mast cells from undergoing apoptosis.
    基因ID
    3562
    UniProt
    P08700
    途径
    JAK/STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy
You are here:
客服