IL-3 抗体 (Biotin)
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- 抗原 See all IL-3 抗体
- IL-3 (Interleukin 3 (IL-3))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This IL-3 antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA
- 纯化方法
- Anti-hIL-3 specific antibody was purified by affinity chromatography and then biotinylated.
- 免疫原
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-3 (human IL-3).
- Top Product
- Discover our top product IL-3 Primary Antibody
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anti-Interleukin 3 (IL-3) antibody
IL-3 适用: 小鼠 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 (IL-3) (AA 101-166) antibodyIL-3 适用: 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 (IL-3) (AA 20-152) antibodyIL-3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 (IL-3) antibodyIL-3 适用: 大鼠 WB, ELISA, Neut 宿主: 山羊 Polyclonal unconjugated
anti-Interleukin 3 (IL-3) (AA 33-166) antibodyIL-3 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 (IL-3) (AA 1-152) antibodyIL-3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 (IL-3) (AA 1-152) antibodyIL-3 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Interleukin 3 (IL-3) (Internal Region) antibodyIL-3 适用: 人, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 (IL-3) antibody (Biotin)IL-3 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal Biotin
anti-Interleukin 3 (IL-3) antibody (Biotin)IL-3 适用: 小鼠 WB, ELISA, Neut 宿主: 兔 Polyclonal Biotin
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- 应用备注
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ELISA:
To detect hIL-3 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-3.
Sandwich:
To detect hIL-3 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human IL-3 (XP-5185) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-3.
Western Blot:
To detect hIL-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 储存条件
- -20 °C
- 储存方法
- IL-3 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- 抗原
- IL-3 (Interleukin 3 (IL-3))
- 别名
- IL-3 (IL-3 产品)
- 别名
- BPA antibody, Csfmu antibody, HCGF antibody, Il-3 antibody, MCGF antibody, PSF antibody, IL-3 antibody, MULTI-CSF antibody, interleukin 3 antibody, interleukin 3 (colony-stimulating factor, multiple) antibody, Il3 antibody, IL3 antibody
- 背景
- The pleiotropic IL-3 (Interleukin 3) is a 15 kDa cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL-3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells. IL-3 not only supports growth of both pluripotent stem cells and the more differentiated committed progenitors, but it also stimulates the functional activity of some fully differentiated cells. IL-3 has also been shown to protect mast cells from undergoing apoptosis.
- 基因ID
- 3562
- UniProt
- P08700
- 途径
- JAK/STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy
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