Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
特异性
This antibody is expected to recognize both reported isoforms (NP_004090.4, NP_937837.1).
纯化方法
Stomatin Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
Stomatin Antibody was raised against synthetic peptide EKRHTRDSEAQR found near the N Terminus of Stomatin.
ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 30 kDa band observed in Human Erythrocytes, Spleen, Bone Marrow and Lung lysates (calculated MW of 31.7 kDa according to NP_004090.4). Recommended concentration: 0.1-0.3μ,g/mL. Immunohistochemistry: In paraffin embedded Human Liver shows membrane staining of hepatocytes and of sinusoidal lining cells. Recommended concentration, 5-10μ,g/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Stomatin is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Aliquot and store at -20°C. Minimize freezing and thawing.