Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
纯化方法
SFRP4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
SFRP4 antibody was raised against a 15 amino acid synthetic peptide near the internal region of SFRP4.
ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 40 kDa band observed in Human Uterus lysates (calculated MW of 39.8 kDa according to NP_003005.2). Recommended concentration: 1-3μ,g/mL. Immunohistochemistry: In paraffin embedded Human Uterus shows granular staining in the apical cytoplasm of the endometrial gland. Recommended concentration, 2-4μ,g/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
SFRP4 antibody is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Aliquot and store at -20°C. Minimize freezing and thawing.
抗原
SFRP4
(Secreted Frizzled-Related Protein 4 (SFRP4))