Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
特异性
This antibody is expected to recognize isoform 1 (NP_997700.1) only.
纯化方法
PRKCB Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
PRKCB Antibody was raised against synthetic peptide DKEFTRQPVELT found at an internal region (near the C Terminus) of PRKCB.
ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 80 kDa band observed in lysates of Human Placenta and Rat Brain (calculated MW of 76.9 kDa according to NP_997700.1). Recommended concentration: 0.1-0.3μ,g/mL. Immunohistochemistry: In paraffin embedded Human Spleen shows nucleolar staining in some splenocytes. Recommended concentration, 1-2μ,g/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
PRKCB is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Aliquot and store at -20°C. Minimize freezing and thawing.