Western Blotting (WB), ELISA, Enzyme Immunoassay (EIA)
纯化方法
cytokeratin 20 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
cytokeratin 20 Antibody was raised against synthetic peptide NSRHTVNYGSDLT found at an internal region of cytokeratin 20.
ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 49 kDa band observed in Human Colon and Ileum lysates (calculated MW of 48.5 kDa according to NP_061883.1). Recommended concentration: 0.01-0.03μ,g/mL. Enzyme immunoassay:This antibody was reported to work in sandwich-type ELISA as a capture antibody.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
cytokeratin 20 is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Aliquot and store at -20°C. Minimize freezing and thawing.