This antibody is expected to recognize all reported isoform 1 (NP_001659.1) only.
纯化方法
ARNT Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
ARNT Antibody was raised against synthetic peptide CNDKERFARSDDEQS found at an internal region (near the N Terminus) of ARNT.
ELISA: antibody detection limit dilution 1:4000. Western Blot: Approx 90 kDa band observed in nuclear lysates of cell lines HeLa and HepG2 (calculated MW of 86.6 kDa according to NP_001659.1). Recommended concentration: 2-4μ,g/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
ARNT is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Aliquot and store at -20°C. Minimize freezing and thawing.