Collagen IV 抗体
Quick Overview for Collagen IV 抗体 (ABIN5680242)
抗原
See all Collagen IV (COL4) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Human Collagen IV, no cross-reactivity with other Collagen types, Vitronectin, Fibronectin or Chondroitinsulfate Human and other primates, no reactivity in chicken
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无交叉反应
- Cat, 小鸡, 山羊, 兔, 大鼠, 绵羊
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交叉反应 （详细）
- Human and other primates (no reactivity in chicken, cat, rabbit, rat, sheep and goat)
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产品特性
- Type IV Collagen is a non-fibrillary network of different alpha-chains: alpha 1(IV) to-alpha 6(IV). It is typically found in basal membranes of different organs (e.g. skin, lens, lung, kidney). Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Human Collagen IV, no cross-reactivity with other Collagen types, Vitronectin, Fibronectin or Chondroitinsulfate.
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纯化方法
- Antibody solution
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免疫原
- Purified human Collagen IV
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亚型
- IgG1
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anti-Collagen, Type IV (COL4) antibody
COL4 适用: 人, Cow WB, ELISA, IHC, IP, DB, FM 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) (AA 1571-1669) antibodyCOL4 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, IHC (fro), IHC (p), FACS, IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) (AA 1401-1500) antibodyCOL4 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, IHC (fro), IHC (p), FACS, IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人 ELISA, IHC, IHC (fro), IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-704R unconjugated Recombinant Antibody
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人, 大鼠, Pig, Cow WB, IHC, IHC (fro), IHC (p), ICC 宿主: 小鼠 Monoclonal LP46 unconjugated
anti-Collagen, Type IV (COL4) antibody (FITC)COL4 适用: 人 ELISA 宿主: 小鼠 Monoclonal 2F11 FITC
anti-Collagen, Type IV (COL4) antibody (Biotin)COL4 适用: 人 ELISA 宿主: 山羊 Polyclonal Biotin
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应用备注
- After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Human skin, placenta or lens capsule,IHC(C,P), WB,60 min at RT,(liquid conc.) 1:10 - 1:50
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide. The volume is sufficient for at least 100 immunohistochemical tests (100 μL working solution / test)
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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- Collagen IV (COL4) (Collagen, Type IV (COL4))
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别名
- Collagen Type IV
抗原
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