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c-FOS 抗体 (AA 280-380) (PE)

Name: c-FOS 抗体 (AA 280-380) (PE)
Brand: antibodies-online
SKU: ABIN5650659
Availability: OutOfStock

c-Fos 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal 4A3 PE

Western Blot analysis of Human Serum starved HeLa cells treated with PMA showing detection of 41 kDa c-fos protein using Mouse Anti-c-fos Monoclonal Antibody, Clone 4A3 (ABIN5650659). (c-FOS 抗体 (AA 280-380) (PE))

Immunohistochemistry analysis using Mouse Anti-c-Fos Monoclonal Antibody, Clone 4A3 (ABIN5650659). (c-FOS 抗体 (AA 280-380) (PE))

IHC

2 images

产品编号 ABIN5650659

发货至: 中国

Datasheet as PDF

抗原 See all c-FOS (c-Fos) 抗体

c-FOS (c-Fos) (c-Fos)
抗原表位
26

25

20

19

17

16

15

15

10

8

4

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 280-380
适用
200

155

136

13

12

11

8

7

6

4

4

3

3

2

1
人
宿主
192

35

6

1
小鼠
Compatible Secondaries
克隆类型
196

37
单克隆
标记
106

14

12

8

8

8

8

8

8

8

6

6

6

6

6

6

4

2

2

2
This c-FOS antibody is conjugated to PE
应用范围
200

80

80

76

39

33

33

25

13

12

12

6

5

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC)

特异性

Expressed at very low levels in quiescent cells. When cells are stimulated to reenter growth, they undergo 2 waves of expression, the first one peaks 7.5 minutes following FBS induction. At this stage, the protein is localized endoplasmic reticulum. The second wave of expression occurs at about 20 minutes after induction and peaks at 1 hour. At this stage, the protein becomes nuclear.,Detects 62 kDa band. Observed molecular weight of band is higher than predicted 41 kDa band due to post translational modifications.

交叉反应

人, 大鼠

纯化方法

Protein G Purified

免疫原

Synthetic peptide from the N-terminal of Human c-Fos (aa. 280-380)

克隆位点

4A3

亚型

IgG2

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应用备注
- WB (1:1000)
- optimal dilutions for assays should be determined by the user.
说明

A 1:1000 dilution of ABIN5650659 was sufficient for detection of c-fos in 10 μg of serum starved HeLa cells treated with PMA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Conjugated antibodies should be stored at 4°C
抗原

c-FOS (c-Fos) (c-Fos)

别名

c-fos (c-Fos 产品)

别名

AP-1 antibody, C-FOS antibody, p55 antibody, c-fos antibody, D12Rfj1 antibody, cFos antibody, cFOS antibody, fos antibody, Fos proto-oncogene, AP-1 transcription factor subunit antibody, FBJ osteosarcoma oncogene antibody, proto-oncogene c-Fos-like antibody, FOS antibody, Fos antibody, LOC101078177 antibody

基因ID

2353

NCBI登录号

NP_005243

UniProt

P01100

途径

S100 Proteins