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c-FOS 抗体 (pThr325)

Name: c-FOS 抗体 (pThr325)
SKU: ABIN684928
Availability: OutOfStock

c-Fos 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), FACS, IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated

Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Anti-Phospho-c-Fos (Thr325) Polyclonal Antibody, Unconjugated (ABIN684928) at 1:200 followed by conjugation to the secondary antibody and DAB staining. (c-FOS 抗体 (pThr325))

IHC

1 image

(1 reference)

产品编号 ABIN684928

发货至: 中国

Datasheet as PDF

抗原 See all c-FOS (c-Fos) 抗体

c-FOS (c-Fos) (c-Fos)
抗原表位
26

25

25

19

18

15

15

15

11

8

4

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pThr325
适用
210

154

135

13

12

11

8

7

6

4

4

3

3

2

2
人, 小鼠, 大鼠
宿主
200

36

8

1
兔
Compatible Secondaries
克隆类型
197

47
多克隆
标记
112

14

12

10

8

8

8

8

8

8

8

6

6

6

6

6

5

2

2

2
This c-FOS antibody is un-conjugated
应用范围
204

79

79

76

41

39

33

25

13

12

12

6

6

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

特异性

This phosphorylation site is homologous to Thr325 in Mouse and Rat.

交叉反应

人, 小鼠, 大鼠

预测反应

Dog,Sheep,Pig,Chicken

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic phosphopeptide derived from human c-Fos around the phosphorylation site of Thr325

亚型

IgG

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应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Tavares, Pathak: "Helicobacter pylori Secreted Protein HP1286 Triggers Apoptosis in Macrophages via TNF-Independent and ERK MAPK-Dependent Pathways." in: Frontiers in cellular and infection microbiology, Vol. 7, pp. 58, (2017) (PubMed).
抗原

c-FOS (c-Fos) (c-Fos)

别名

c-Fos (c-Fos 产品)

别名

AP-1 antibody, C-FOS antibody, p55 antibody, c-fos antibody, D12Rfj1 antibody, cFos antibody, cFOS antibody, fos antibody, Fos proto-oncogene, AP-1 transcription factor subunit antibody, FBJ osteosarcoma oncogene antibody, proto-oncogene c-Fos-like antibody, FOS antibody, Fos antibody, LOC101078177 antibody

背景

Synonyms: p55, AP-1, C-FOS, Proto-oncogene c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7, FOS, G0S7
Background: Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.

基因ID

2353

UniProt

P01100

途径

S100 Proteins