c-FOS 抗体 (AA 280-380) (HRP)
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- 抗原 See all c-FOS (c-Fos) 抗体
- c-FOS (c-Fos) (c-Fos)
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抗原表位
- AA 280-380
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This c-FOS antibody is conjugated to HRP
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应用范围
- Western Blotting (WB)
- 特异性
- Expressed at very low levels in quiescent cells. When cells are stimulated to reenter growth, they undergo 2 waves of expression, the first one peaks 7.5 minutes following FBS induction. At this stage, the protein is localized endoplasmic reticulum. The second wave of expression occurs at about 20 minutes after induction and peaks at 1 hour. At this stage, the protein becomes nuclear.,Detects 62 kDa band. Observed molecular weight of band is higher than predicted 41 kDa band due to post translational modifications.
- 交叉反应
- 人
- 纯化方法
- Protein G Purified
- 免疫原
- Synthetic peptide from the N-terminal of Human c-Fos (aa. 280-380)
- 克隆位点
- 3G11
- 亚型
- IgG1
- Top Product
- Discover our top product c-Fos Primary Antibody
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- 应用备注
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- WB (1:1000)
- optimal dilutions for assays should be determined by the user.
- 说明
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A 1:1000 dilution of ABIN5650643 was sufficient for detection of c-fos in 10 μg of serum starved HeLa cells treated with PMA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Conjugated antibodies should be stored at 4°C
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- 抗原
- c-FOS (c-Fos) (c-Fos)
- 别名
- c-fos (c-Fos 产品)
- 别名
- AP-1 antibody, C-FOS antibody, p55 antibody, c-fos antibody, D12Rfj1 antibody, cFos antibody, cFOS antibody, fos antibody, Fos proto-oncogene, AP-1 transcription factor subunit antibody, FBJ osteosarcoma oncogene antibody, proto-oncogene c-Fos-like antibody, FOS antibody, Fos antibody, LOC101078177 antibody
- 基因ID
- 2353
- NCBI登录号
- NP_005243
- UniProt
- P01100
- 途径
- S100 Proteins
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