Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
原理
Rabbit polyclonal antibody raised against partial recombinant PML.
特异性
This antibody recognizes normal PML proteins of lower molecular weight, and also recognizes the high molecular weight PML/RARalpha chimeric protein in promyelocytic leukemia patients.
交叉反应
人
免疫原
Recombinant protein corresponding to the ring finger in the homodimerization region of human PML.
Western Blot (1:5000) Immunofluorescence (1:500) Immunohistochemistry (1:100) The optimal working dilution should be determined by the end user.
限制
仅限研究用
状态
Lyophilized
缓冲液
Lyophilized from 10 mM PBS, pH 7.4
储存条件
-20 °C
储存方法
Store at -20°C on dry atmosphere. After reconstitution with appropriate volume of deionized water to reach a final antibody concentration of 1 mg/mL, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.