Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
原理
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human GSK3A.
特异性
GSK3A (phospho S21) polyclonal antibody detects endogenous level of GSK3A only when phosphorylated at serine 21 and does not detect GSK3B when phosphorylated at serine 9.
交叉反应
人, 小鼠, 兔
免疫原
A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S21 of human GSK3A.
GSK3a
适用: 人
WB, ELISA, IF
宿主: 兔
Polyclonal
unconjugated
应用备注
Immunofluorescence (1:100-1:200) Immunohistochemistry (1:50-1:100) Western Blot (1:500-1:1000) The optimal working dilution should be determined by the end user.
限制
仅限研究用
状态
Liquid
缓冲液
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50 % glycerol, 0.02 % sodium azide)
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C,-20 °C
储存方法
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.