Collagen IV 抗体
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北京 101111
Quick Overview for Collagen IV 抗体 (ABIN5541082)
抗原
See all Collagen IV (COL4) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody clone 1043 recognizes type IV collagen in the extracellular - basement membranes of normal and pathological tissues.
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交叉反应 （详细）
- Does NOT react with Rat and Rabbit.
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纯化方法
- Purified
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免疫原
- Purified Human type IV Collagen, isolated from Human placenta. Antibody derived by fusion of Sp 2/0 mouse myeloma cells with spleen cells from a BALB/c mouse.
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亚型
- IgG1
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anti-Collagen, Type IV (COL4) antibody
COL4 适用: 人, Cow WB, ELISA, IHC, IP, DB, FM 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) (AA 1571-1669) antibodyCOL4 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, IHC (fro), IHC (p), FACS, IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人, 小鼠, 大鼠 IHC (p), IF 宿主: 小鼠 Monoclonal 8E5 unconjugated
anti-Collagen, Type IV (COL4) (AA 1401-1500) antibodyCOL4 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, IHC (fro), IHC (p), FACS, IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人 IHC 宿主: 兔 Monoclonal 704R unconjugated Recombinant Antibody
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人 ELISA, IHC, IHC (fro), IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type IV (COL4) antibodyCOL4 适用: 人, 大鼠, Pig, Cow WB, IHC, IHC (fro), IHC (p), ICC 宿主: 小鼠 Monoclonal LP46 unconjugated
anti-Collagen, Type IV (COL4) antibody (FITC)COL4 适用: 人 ELISA 宿主: 小鼠 Monoclonal 2F11 FITC
anti-Collagen, Type IV (COL4) antibody (Biotin)COL4 适用: 人 ELISA 宿主: 山羊 Polyclonal Biotin
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应用备注
- Western blot : 1/100-1/1000. Immunocytochemistry on fixed cells (methanol fixation). Flow cytometry. Immunohistochemistry on Frozen Sections using a PBS buffer containing 0.1 mM CaCl 2 and 0.1 mM MgCl. Immunohistochemistry on Paraffin Sections : Pretreatement with 1 mg/mL pepsin in 0.01N HCl for 30 minutes at room temperature is recommended. Recommended Dilutions : 1/50-1/100 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Buffer System: PBS Preservatives: 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles. Shelf life: One year from despatch.
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- Collagen IV (COL4) (Collagen, Type IV (COL4))
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别名
- collagen type iv
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背景
- Type IV collagen is an integral part of most, if not all basement membranes. The extent of expression of the basement membrane component type IV collagen may be correlated with the degree of differentiation of basement membrane producing neoplasms. Neoplastic cells of invasive carcinomas often lack a continuous basement membrane. In diagnostic histopathology antibodies to type IV collagen may help to distinguish between non-invasive (benign or in situ) and invasive lesions. Furthermore the antiserum can be of help in the differential diagnosis of bullous lesions in dermatopathology.
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UniProt
- P02462
抗原
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