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CD14 抗体 (AA 16-260)

This anti-CD14 antibody is a 兔 多克隆 antibody detecting CD14 in FACS, WB 和 IHC. Suitable for 小鼠 和 大鼠. This Primary Antibody has been cited in 4+ publications.
Pubmed (4 references)
产品编号 ABIN5518974
发货至: 中国

Quick Overview for CD14 抗体 (AA 16-260) (ABIN5518974)

抗原

See all CD14 抗体
CD14

适用

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小鼠, 大鼠

宿主

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兔

克隆类型

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多克隆

标记

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This CD14 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC)
Discover our top product CD14 Primary Antibody

  • 抗原表位

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    AA 16-260

    原理

    Anti-CD14 Antibody Picoband®

    交叉反应 （详细）

    No cross-reactivity with other proteins.

    产品特性

    Anti-CD14 Antibody Picoband® (ABIN5518974). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    纯化方法

    Immunogen affinity purified.

    免疫原

    E. coli-derived mouse CD14 recombinant protein (Position: S16-A260).

    亚型

    IgG
  • anti-CD14 (CD14) (AA 20-214) antibody
    anti-CD14 (CD14) (AA 20-214) antibody

    CD14 适用: 人 IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 1H5D8 unconjugated

    anti-CD14 (CD14) (AA 20-214) antibody
    anti-CD14 (CD14) (AA 20-214) antibody

    CD14 适用: 人, 小鼠 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 4B4F12 unconjugated

    anti-CD14 (CD14) (AA 20-345) antibody
    anti-CD14 (CD14) (AA 20-345) antibody

    CD14 适用: 人 FACS, WB, IHC, ELISA, IF 宿主: 小鼠 Monoclonal 14G1F3 unconjugated

    anti-CD14 (CD14) antibody
    anti-CD14 (CD14) antibody

    CD14 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 1A3B6 unconjugated

    anti-CD14 (CD14) (AA 25-148) antibody
    anti-CD14 (CD14) (AA 25-148) antibody

    CD14 适用: 人 FACS, WB, IHC, ISt, StM 宿主: 小鼠 Monoclonal LPSR-2385 unconjugated

    anti-CD14 (CD14) (AA 230-345) antibody
    anti-CD14 (CD14) (AA 230-345) antibody

    CD14 适用: 人 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 1D1F8 unconjugated

    anti-CD14 (CD14) (AA 54-83), (N-Term) antibody
    anti-CD14 (CD14) (AA 54-83), (N-Term) antibody

    CD14 适用: 人 FACS, WB, IF, IHC (p) 宿主: 兔 Polyclonal RB14105 unconjugated

    anti-CD14 (CD14) (AA 201-300) antibody
    anti-CD14 (CD14) (AA 201-300) antibody

    CD14 适用: 人, 小鼠, 大鼠, 绵羊, 犬, 兔 WB, ELISA, ICC, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

    anti-CD14 (CD14) (Internal Region) antibody
    anti-CD14 (CD14) (Internal Region) antibody

    Verified CD14 适用: 人 FACS, WB, ELISA 宿主: 山羊 Polyclonal unconjugated

    anti-CD14 (CD14) (AA 25-148) antibody
    anti-CD14 (CD14) (AA 25-148) antibody

    CD14 适用: 人 WB, IHC, ISt, StM 宿主: 小鼠 Monoclonal LPSR-2397 unconjugated

  • 应用备注

    Western blot, 0.1-0.5 μg/mL
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells
    1. Goyert, S. M., Ferrero, E., Rettig, W. J., Yenamandra, A. K., Obata, F., Le Beau, M. M. The CD14 monocyte differentiation antigen maps to a region encoding growth factors and receptors. Science 239: 497-500, 1988. 2. LeVan, T. D., Bloom, J. W., Bailey, T. J., Karp, C. L., Halonen, M., Martinez, F. D., Vercelli, D. A common single nucleotide polymorphism in the CD14 promoter decreases the affinity of Sp protein binding and enhances transcriptional activity. J. Immun. 167: 5838-5844, 2001. 3. Wright, S. D. CD14: a leukocyte membrane protein that functions in the response to endotoxin. (Abstract) FASEB J. 4: A1848 only, 1990.

    说明

    We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

    限制

    仅限研究用

  • 状态

    Lyophilized

    溶解方式

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Wu, Yang, Qi, Zhou, Hu, Wang: "ANTI-INFLAMMATORY ACTIVITY OF PLATYCODIN D ON ALCOHOL-INDUCED FATTY LIVER RATS VIA TLR4-MYD88-NF-κB SIGNAL PATH." in: African journal of traditional, complementary, and alternative medicines : AJTCAM, Vol. 13, Issue 4, pp. 176-183, (2017) (PubMed).

    Wu, Liu, Zhang, Xing, Zheng, Ji: "Chinese medicinal formula, Qinggan Huoxue Recipe protects rats from alcoholic liver disease via the lipopolysaccharide-Kupffer cell signal conduction pathway." in: Experimental and therapeutic medicine, Vol. 8, Issue 2, pp. 363-370, (2014) (PubMed).

    Huang, Zhu, Zhang, Zhu, Liu, Zhu, Wang, Li, Yang, Dong, Liu, Chen, Zhang, Yang, Deng, Fan, Wang, Liu, Ma, Fu, Wu: "S100+ cells: a new neuro-immune cross-talkers in lymph organs." in: Scientific reports, Vol. 3, pp. 1114, (2013) (PubMed).

    Zhao, Zhao, Feng, Zhang, Zhuang, Zhao, Li, Li, Liu: "Detection of vulnerable atherosclerotic plaque and prediction of thrombosis events in a rabbit model using 18F-FDG -PET/CT." in: PLoS ONE, Vol. 8, Issue 4, pp. e61140, (2013) (PubMed).

  • 抗原

    CD14

    别名

    Cd14

    背景

    Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14, Cd14,

    Tissue Specificity: Detected on peritoneal macrophages (at protein level) (PubMed:8612135). Cell surface expression detected in lung alveolar macrophages, dendritic macrophages and lung macrophages (at protein level) (PubMed:19362712).

    Background: CD14 is a single-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. This gene is located at bands 5q23-q31. The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.

    分子量

    50 kDa

    基因ID

    12475

    UniProt

    P10810

    途径

    TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
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