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CD14 抗体 (Internal Region)

Name: CD14 抗体 (Internal Region)
Brand: antibodies-online
SKU: ABIN185563
Availability: OutOfStock

Verified CD14 适用: 人 FACS, WB, ELISA 宿主: 山羊 Polyclonal unconjugated

(ABIN185563) Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.

FACS

(ABIN185563) (2 μg/mL) staining of Human Lymph node (A) + peptide (B) and Tonsil (C) + peptide (D) lysate (35 μg protein in RIPA buffer) Detected by chemiluminescence.

ABIN185563 (1µg/ml) staining of Human Lymph Node lysate (35µg protein in RIPA buffer).

ABIN185563 (1µg/ml) staining of PBM lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide.

ABIN185563 (4µg/ml) staining of paraffin embedded Human Spleen.

IHC

5 images

产品编号 ABIN185563

发货至: 中国

Datasheet as PDF

抗原 See all CD14 抗体

CD14
抗原表位
16

9

7

6

5

4

4

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Internal Region
适用
245

47

30

29

28

28

25

24

22

18

15

14

10

9

8

8

8

5

2

2

2

1

1

1
人
宿主
204

63

16

2

1
山羊
Compatible Secondaries
克隆类型
223

63
多克隆
标记
119

29

22

17

13

6

5

4

4

4

4

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD14 antibody is un-conjugated
应用范围
201

97

60

52

46

44

22

21

20

14

13

13

7

4

4

3

3

3

3

2

2

1

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), ELISA

原理

CD14

序列

KRVDADADPR QYAD

交叉反应

人

纯化方法

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

质量等级

Verified

免疫原

Peptide with sequence C-KRVDADADPRQYAD, from the internal region of the protein sequence according to NP_000582.1.

亚型

IgG

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应用备注

Western Blot: Approx 55 kDa band observed in Human Lymph node and Tonsil lysates (calculated MW of 40.1 kDa according to NP_000582.1).The observed molecular weight corresponds to the glycosylated form, and was successfully blocked by incubation with the im
Peptide ELISA: antibody detection limit dilution 1:128000.

说明

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

限制

仅限研究用
状态

Liquid

浓度

0.5 mg/mL

缓冲液

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Minimize freezing and thawing.

储存条件

-20 °C

储存方法

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
抗原

CD14

别名

CD14 (CD14 产品)

别名

CD14 molecule antibody, CD14 antigen antibody, CD14 antibody, Cd14 antibody

背景

CD14, CD14 antigen , HGNC:1628

基因ID

929

NCBI登录号

NP_000582

途径

TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades