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CD14 抗体 (AA 25-148)

CD14 适用: 人 FACS, WB, IHC, ISt, StM 宿主: 小鼠 Monoclonal LPSR-2385 unconjugated
产品编号 ABIN6941093
发货至: 中国
  • 抗原 See all CD14 抗体
    CD14
    抗原表位
    • 16
    • 8
    • 7
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
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    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    • 1
    • 1
    AA 25-148
    适用
    • 246
    • 47
    • 30
    • 29
    • 28
    • 28
    • 25
    • 24
    • 22
    • 18
    • 15
    • 14
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    • 8
    • 8
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    宿主
    • 203
    • 64
    • 16
    • 3
    • 1
    小鼠
    克隆类型
    • 223
    • 64
    单克隆
    标记
    • 120
    • 29
    • 22
    • 17
    • 13
    • 6
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    • 4
    • 4
    • 4
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    • 3
    • 3
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    • 1
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    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD14 antibody is un-conjugated
    应用范围
    • 201
    • 98
    • 61
    • 52
    • 46
    • 45
    • 22
    • 21
    • 20
    • 14
    • 13
    • 13
    • 6
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    • 3
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    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM)
    特异性
    Recognizes a protein of 55 kDa, identified as CD14 (also known lipopolysaccharide receptor). CD14 is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.
    纯化方法
    Purified by Protein A/G
    免疫原
    Recombinant fragment of human CD14 protein (around aa 25-148) (exact sequence is proprietary)
    克隆位点
    LPSR-2385
    亚型
    IgG1 kappa
    Top Product
    Discover our top product CD14 Primary Antibody
  • 应用备注

    Positive Control: A549, THP-1 cells and human monocytes. Lymph nodes and tonsils.

    Known Application: Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    限制
    仅限研究用
  • 浓度
    200 μg/mL
    缓冲液
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-80 °C
    储存方法
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    有效期
    24 months
  • 抗原
    CD14
    别名
    CD14 (CD14 产品)
    别名
    CD14 molecule antibody, CD14 antigen antibody, CD14 antibody, Cd14 antibody
    分子量
    55kDa
    基因ID
    929
    UniProt
    P08571
    途径
    TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
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