CD14 抗体 (AA 25-148)
Our Local Distributor
北京 101111
Quick Overview for CD14 抗体 (AA 25-148) (ABIN6941093)
抗原
See all CD14 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- AA 25-148
-
原理
- CD14 (Monocyte / Macrophage Marker) Antibody
-
特异性
- Recognizes a protein of 55 kDa, identified as CD14 (also known lipopolysaccharide receptor). CD14 is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.
-
纯化方法
- 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.
-
免疫原
- Recombinant fragment of human CD14 protein (around aa 25-148) (exact sequence is proprietary)
-
亚型
- IgG1, kappa
-
-
anti-CD14 (CD14) (AA 20-214) antibody
CD14 适用: 人, 小鼠 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 4B4F12 unconjugated
anti-CD14 (CD14) (AA 20-214) antibodyCD14 适用: 人 IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 1H5D8 unconjugated
anti-CD14 (CD14) antibodyCD14 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 1A3B6 unconjugated
anti-CD14 (CD14) (AA 20-345) antibodyCD14 适用: 人 FACS, WB, IHC, IF, ELISA 宿主: 小鼠 Monoclonal 14G1F3 unconjugated
anti-CD14 (CD14) (AA 230-345) antibodyCD14 适用: 人 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 1D1F8 unconjugated
anti-CD14 (CD14) (AA 65-327) antibodyCD14 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-CD14 (CD14) (AA 54-83), (N-Term) antibodyCD14 适用: 人 FACS, WB, IF, IHC (p) 宿主: 兔 Polyclonal RB14105 unconjugated
anti-CD14 (CD14) (AA 201-300) antibodyCD14 适用: 人, 小鼠, 大鼠, 绵羊, 犬, 兔 WB, ELISA, ICC, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD14 (CD14) (Internal Region) antibodyVerified CD14 适用: 人 FACS, WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-CD14 (CD14) antibody (Biotin)CD14 适用: 人, Non-Human Primate FACS, WB, IP, ELISA 宿主: 小鼠 Monoclonal MEM-18 Biotin
-
-
应用备注
-
Positive Control: A549, THP-1 cells and human monocytes. Lymph nodes and tonsils.
Known Application: Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
-
限制
- 仅限研究用
-
-
-
浓度
- 200 μg/mL
-
缓冲液
- Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C
-
储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
-
有效期
- 24 months
-
-
- CD14
-
别名
- CD14
-
背景
-
Lipopolysaccharide receptor, LPS-R, LPSR, Mo2, Monocyte Differentiation Antigen 14, Myeloid cell-specific leucine-rich glycoprotein
A549, THP-1 cells and human monocytes. Lymph nodes and tonsils.
-
分子量
- 55kDa
-
基因ID
- 929, 163867
-
UniProt
- P08571
-
途径
- TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
抗原
-
