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Histone 3 抗体 (AA 1-50)

Name: Histone 3 抗体 (AA 1-50)
SKU: ABIN5004684
Availability: OutOfStock

This anti-Histone 3 antibody is a 小鼠单克隆 antibody detecting Histone 3 in WB, IHC (p) 和 IF (p). Suitable for 人, 小鼠和大鼠. This Primary Antibody has been cited in 2+ publications.

K562 cell lysates; probed with Histone H3/HIST3H3 (3G1) Monoclonal Antibody, unconjugated (bsm-33042M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C. (Histone 3 抗体 (AA 1-50))

Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Histone H3/HIST3H3 (3G1) Monoclonal Antibody, Unconjugated (bsm-33042M)at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining. (Histone 3 抗体 (AA 1-50))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Histone H3/HIST3H3 (3G1) Monoclonal Antibody, Unconjugated (bsm-33042M)at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining. (Histone 3 抗体 (AA 1-50))

IHC (p)

HeLa cell lysates; probed with Histone H3/HIST3H3 (3G1) Monoclonal Antibody, unconjugated (bsm-33042M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C. (Histone 3 抗体 (AA 1-50))

4 images

(2 references)

产品编号 ABIN5004684

发货至: 中国

Quick Overview for Histone 3 抗体 (AA 1-50) (ABIN5004684)

抗原

See all Histone 3 (H3) 抗体

Histone 3 (H3) (Histone H3 (H3))

适用

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人, 小鼠, 大鼠

宿主

小鼠

克隆类型

单克隆

标记

This Histone 3 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

克隆位点

3G1

Discover our top product H3 Primary Antibody

抗原表位
52

50

48

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39

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31

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23

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8
AA 1-50

交叉反应

人, 小鼠, 大鼠

纯化方法

Purified by Protein G.

免疫原

Recombinant human HIST3H3 Protein

亚型

IgG
anti-Histone H3 (H3) (H3K4me) antibody
H3 适用: 人 WB, IF, ChIP, IP, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27ac) antibody
H3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (3meLys4) antibody
H3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9ac) antibody
H3 适用: 人, 小鼠 WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me3) antibody
H3 适用: 人, 小鼠 WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 小鼠 Monoclonal MABI 0333 unconjugated

anti-Histone H3 (H3) (AA 71-136) antibody
H3 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K4me3) antibody
H3 适用: 人, 小鼠, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (C-Term) antibody
H3 适用: 人 WB, IHC, ChIP, IP 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9me2) antibody
H3 适用: 人 WB, IF, IHC, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3R2me2) antibody
H3 适用: 人 WB, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Tian, Wu, Guo, Ma, Huang, Zhao: "Minocycline attenuates sevoflurane-induced cell injury via activation of Nrf2." in: International journal of molecular medicine, Vol. 39, Issue 4, pp. 869-878, (2017) (PubMed).

Zhao, Ding, Liu, Yin, Zhang, Ma: "Unfractionated heparin protects the protein C system against lipopolysaccharide-induced damage in vivo and in vitro." in: Experimental and therapeutic medicine, Vol. 14, Issue 6, pp. 5515-5522, (2017) (PubMed).
抗原

Histone 3 (H3) (Histone H3 (H3))

别名

Histone H3

背景

Synonyms: Histone Cluster 3, H3, H3 Histone Family, Member T, Histone 3, H3, H3FT, H3/G, H3/T, H3t, H3.4 , Histone H3.1t , HIST3H3, HGNC:4778, H31T_HUMAN
Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

基因ID

8290

UniProt

Q16695