我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

Histone 3 抗体 (AA 1-50)

Name: Histone 3 抗体 (AA 1-50)
SKU: ABIN5004684
Availability: OutOfStock

H3 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF (p) 宿主: 小鼠 Monoclonal 3G1 unconjugated

K562 cell lysates; probed with Histone H3/HIST3H3 (3G1) Monoclonal Antibody, unconjugated (bsm-33042M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C. (Histone 3 抗体 (AA 1-50))

Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Histone H3/HIST3H3 (3G1) Monoclonal Antibody, Unconjugated (bsm-33042M)at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining. (Histone 3 抗体 (AA 1-50))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Histone H3/HIST3H3 (3G1) Monoclonal Antibody, Unconjugated (bsm-33042M)at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining. (Histone 3 抗体 (AA 1-50))

IHC (p)

HeLa cell lysates; probed with Histone H3/HIST3H3 (3G1) Monoclonal Antibody, unconjugated (bsm-33042M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C. (Histone 3 抗体 (AA 1-50))

4 images

(2 references)

产品编号 ABIN5004684

发货至: 中国

Datasheet as PDF

抗原 See all Histone 3 (H3) 抗体

Histone 3 (H3)
抗原表位
56

47

44

41

40

36

35

35

34

34

34

32

29

29

28

27

26

25

23

22

22

21

20

18

18

17

16

16

16

16

16

16

15

14

14

13

13

12

12

12

11

11

11

10

10

10

10

9

9

8
AA 1-50
适用
1368

938

859

59

42

41

41

40

39

35

35

29

26

22

19

6

3

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
人, 小鼠, 大鼠
宿主
1184

241

8

4
小鼠
Compatible Secondaries
克隆类型
1109

327

1
单克隆
标记
795

81

63

63

63

62

62

62

34

33

21

17

17

17

17

17

12

1
This Histone 3 antibody is un-conjugated
应用范围
1034

402

373

311

240

188

175

166

162

151

137

127

116

48

38

34

32

20

16

14

7

7

6

4

3

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

交叉反应

人, 小鼠, 大鼠

纯化方法

Purified by Protein G.

免疫原

Recombinant human HIST3H3 Protein

克隆位点

3G1

亚型

IgG

Top Product

Discover our top product H3 Primary Antibody
anti-Histone 3 (H3) (H3K4me) antibody
H3 适用: 人 WB, IF, ChIP, IP, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone 3 (H3) (H3K27ac) antibody
H3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone 3 (H3) (3meLys4) antibody
H3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone 3 (H3) (H3K36me3) antibody
H3 适用: 人, 小鼠 WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 小鼠 Monoclonal MABI 0333 unconjugated

anti-Histone 3 (H3) (H3K9ac) antibody
H3 适用: 人, 小鼠 WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone 3 (H3) (AA 71-136) antibody
H3 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Histone 3 (H3) (H3K27me) antibody
H3 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-Histone 3 (H3) (H3K4me3) antibody
H3 适用: 人, 小鼠, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone 3 (H3) (C-Term) antibody
H3 适用: 人 WB, IHC, ChIP, IP 宿主: 兔 Polyclonal unconjugated

anti-Histone 3 (H3) (H3K4me3) antibody
H3 适用: 人 WB, IHC, IF, ChIP, IP, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Tian, Wu, Guo, Ma, Huang, Zhao: "Minocycline attenuates sevoflurane-induced cell injury via activation of Nrf2." in: International journal of molecular medicine, Vol. 39, Issue 4, pp. 869-878, (2017) (PubMed).

Zhao, Ding, Liu, Yin, Zhang, Ma: "Unfractionated heparin protects the protein C system against lipopolysaccharide-induced damage in vivo and in vitro." in: Experimental and therapeutic medicine, Vol. 14, Issue 6, pp. 5515-5522, (2017) (PubMed).
抗原

Histone 3 (H3)

别名

Histone H3 (H3 产品)

别名

H-3 antibody, histocompatibility 3 antibody, H3 antibody

背景

Synonyms: Histone Cluster 3, H3, H3 Histone Family, Member T, Histone 3, H3, H3FT, H3/G, H3/T, H3t, H3.4 , Histone H3.1t , HIST3H3, HGNC:4778, H31T_HUMAN
Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

基因ID

8290

UniProt

Q16695