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- 抗原 See all BUB1B 抗体
- BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This BUB1B antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 特异性
- This antibody detects endogenous levels of ubR1 protein. (region surrounding Thr368)
- 交叉反应 （详细）
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Species reactivity (expected):Mouse.
Species reactivity (tested):Human. - 纯化方法
- Affinity chromatography
- 纯度
- > 95 % by SDS-PAGE
- Top Product
- Discover our top product BUB1B Primary Antibody
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anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (AA 1-130) antibody
BUB1B 适用: 人 WB, ELISA, IHC (p), PLA, RNAi 宿主: 小鼠 Monoclonal 2G5 unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) antibodyBUB1B 适用: 人, 大鼠, 猴, 犬 WB, IF, FACS 宿主: 小鼠 Monoclonal 6E3 unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) antibodyBUB1B 适用: 人, 猴 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 11G2 unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (AA 1-130) antibodyBUB1B 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2G9 unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (AA 230-394) antibodyBUB1B 适用: 人 IHC, ELISA, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (C-Term) antibodyBUB1B 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (AA 1-130) antibodyBUB1B 适用: 人 WB, IHC, ELISA, IHC (p), PLA, RNAi 宿主: 小鼠 Monoclonal 2G5 unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (AA 274-525) antibodyBUB1B 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) antibodyBUB1B 适用: 人, 小鼠 WB, IF, IP, FACS, ICC 宿主: 小鼠 Monoclonal 8G1 unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (AA 751-850) antibodyBUB1B 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1,0 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))
- 别名
- BUB1B (BUB1B 产品)
- 别名
- BUB1beta antibody, BUBR1 antibody, Bub1A antibody, MAD3L antibody, MVA1 antibody, SSK1 antibody, hBUBR1 antibody, AU045529 antibody, bub1b antibody, BUB1 antibody, BUB1-like antibody, BubR1 antibody, BubRI antibody, CG14030 antibody, CG14030/Bub1 antibody, Dmel\\CG14030 antibody, bub1 antibody, BUB1 mitotic checkpoint serine/threonine kinase B antibody, BUB1B, mitotic checkpoint serine/threonine kinase antibody, BUB1B, mitotic checkpoint serine/threonine kinase L homeolog antibody, Mitotic checkpoint serine/threonine-protein kinase BUB1 beta antibody, Bub1 kinase antibody, BUB1B antibody, Bub1b antibody, bub1b.L antibody, bub1b antibody, Bub1 antibody
- 背景
- The N-end rule relates the in vivo half-life of a protein to the composition of its N-terminal residues. The N-end rule pathway is part of the ubiquitin system, which involves a three-step mechanism. Proteins targeted for degradation are bound on their N-terminal residue by Ubr1 (also designated E3 α and N-recognin), which catalyzes the covalent attachment of ubiquitin to the protein substrate. Two zinc finger domains and the RING-H2 finger domain of Ubr1 are essential for substrate recognition. Ubr1 is located on mouse chromosome 2 and on human chromosome 15 in the syntenic region. Ubr1 is a 220 kDa protein that is ubiquitously expressed in adult mouse, with the highest expression detected in skeletal muscle and heart. In mouse embryo, Ubr1 is primarily expressed in the branchial arches and in the tail and limb buds.Synonyms: BUB1 beta, BUBR1, MAD3/BUB1-related protein kinase, MAD3L, Mitotic checkpoint kinase MAD3L, Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, Protein SSK1
- 分子量
- approx. 130 kDa
- 基因ID
- 701
- NCBI登录号
- NP_001202
- UniProt
- O60566
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