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- 抗原 See all IKK alpha (CHUK) 抗体
- IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This IKK alpha antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- This antibody detects endogenous levels of IKKα/β protein.
- 交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - 纯化方法
- Affinity chromatography using epitope-specific immunogen
- 纯度
- > 95 % (by SDS-PAGE)
- Top Product
- Discover our top product CHUK Primary Antibody
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anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (C-Term) antibody
CHUK 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (pSer176), (pSer177) antibodyCHUK 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibodyCHUK 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (AA 646-745) antibodyCHUK 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 4B8 unconjugated
anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (AA 646-745) antibodyCHUK 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 1F8 unconjugated
anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (pThr23) antibodyCHUK 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibodyCHUK 适用: 人 WB, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 3G12H9 unconjugated
anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (pThr23) antibodyCHUK 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibodyCHUK 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB2159 unconjugated
anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (N-Term) antibodyCHUK 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
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- 抗原
- IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))
- 别名
- IKKA (CHUK 产品)
- 别名
- ikk antibody, ikk1 antibody, ikka antibody, ikkalpha antibody, CHUK antibody, IKBKA antibody, IKK-alpha antibody, IKK1 antibody, IKKA antibody, NFKBIKA antibody, TCF16 antibody, AI256658 antibody, Chuk1 antibody, Fbx24 antibody, Fbxo24 antibody, Ikka antibody, zgc:56539 antibody, conserved helix-loop-helix ubiquitous kinase S homeolog antibody, conserved helix-loop-helix ubiquitous kinase antibody, chuk.S antibody, CHUK antibody, Chuk antibody, chuk antibody
- 背景
- The transcription factor NFκB is retained in the cytoplasm in an inactive form by the inhibitory protein IκB. Activation of NFκB requires that IκB be phosphorylated on specific serine residues, which results in targeted degradation of IκB. IκB kinase α (IKKα), previously designated CHUK, interacts with IκBα and specifically phosphorylates IκBα on Serine 32 and 36, the sites that trigger its degradation. IKKα appears to be critical for NFκB activation in response to proinflammatory cytokines. Phosphorylation of IκB by IKKα is stimulated by the NFκB inducing kinase (NIK), which itself is a central regulator for NFκB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKα, IKKβ and IKKγ (also designated NEMO), and each appear to make essential contributions to IκB phosphorylation.Synonyms: CHUK, Conserved helix-loop-helix ubiquitous kinase, I kappa-B kinase alpha, I-kappa-B kinase 1, IKK-A, IKK-alpha, IKK1, IkBKA, IkappaB kinase, Inhibitor of nuclear factor kappa-B kinase subunit alpha, NFKBIKA, Nuclear factor NF-kappa-B inhibitor kinase alpha, TCF16
- 分子量
- approx. 85 kDa
- 基因ID
- 1147
- NCBI登录号
- NP_001269
- UniProt
- O15111
- 途径
- PI3K-Akt Signaling, NF-kappaB Signaling, RTK signaling, TCR Signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins
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