Recombinant 5-Methylcytosine 抗体
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- 抗原
- 5-Methylcytosine
- 抗体类型
- Recombinant Antibody
- 适用
- 请咨询
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宿主
- 兔
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Methylated DNA Immunoprecipitation (MeDIP), Dot Blot (DB), Immunocytochemistry (ICC), ELISA, Immunohistochemistry (IHC)
- 产品特性
- This recombinant 5mC antibody reacts to 5-methylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and hydroxymethylcytosine in DNA.
- 纯化方法
- Protein A purified from animal origin-free supernatant
- 免疫原
- BSA-conjugated 5-methylcytosine was used as the immunogen for this recombinant 5mC antibody.
- 克隆位点
- RM231
- 亚型
- IgG
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anti-5-Methylcytosine antibody
MeDIP, DB, ICC, ELISA, IHC (p) 宿主: 兔 Monoclonal RM231 unconjugated
anti-5-Methylcytosine antibody适用: 人 MeDIP, IF, DB 宿主: 小鼠 Monoclonal A1 unconjugated
anti-5-Methylcytosine antibody (Biotin)IF, ICC 宿主: 小鼠 Monoclonal 5MC-CD Biotin
anti-5-Methylcytosine antibody (FITC)IF, ICC 宿主: 小鼠 Monoclonal 5MC-CD FITC
anti-5-Methylcytosine antibodyIF, ICC 宿主: 小鼠 Monoclonal 5MC-CD unconjugated
anti-5-Methylcytosine antibodyWB, FACS, IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-5-Methylcytosine antibodyIF, ICC 宿主: 小鼠 Monoclonal 5MC-CD unconjugated
anti-5-Methylcytosine antibodyMeDIP, FACS, IF, DB, IP, ELISA 宿主: 小鼠 Monoclonal b unconjugated
anti-5-Methylcytosine antibodyWB, MeDIP, FACS, IF, DB, ICC, ELISA, IHC 宿主: 小鼠 Monoclonal 33D3 unconjugated
anti-5-Methylcytosine antibodyMeDIP, DB, MeDIP-Seq 宿主: 小鼠 Monoclonal A1 unconjugated
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- 应用备注
- The stated application concentrations are suggested starting points. Titration of the recombinant 5mC antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Dot Blot: 0.5-2 μg/mL,ELISA: 0.1-1 μg/mL,Immunocytochemistry: 0.5-2 μg/mL,MeDIP: 0.2 μg/mL- 2 μg/mL
- 限制
- 仅限研究用
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- 缓冲液
- 1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store the recombinant 5mC antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).
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- 抗原
- 5-Methylcytosine
- 别名
- 5-Methylcytosine (5mC)
- 物质类
- Chemical
- 背景
- This recombinant 5mC antibody reacts to 5-methylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and hydroxymethylcytosine in DNA.
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