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- 抗原
- 5-Methylcytosine
- 适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Methylated DNA Immunoprecipitation (MeDIP), Immunofluorescence (IF), Dot Blot (DB)
- 产品特性
- 5-Methylcytosine (5-mC) is a modified base that is found in the DNA of plants and vertebrates. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. The 5-methylcytidine antibody (Clone A1) has been developed to discriminate between the modified base and its normal cytosine counterpart, allowing for gene promoter methylation analysis. 5-Methylcytosine (5-mC) antibody (mAb) (Clone A1) was raised in a Mouse host. It has been validated for use in Dot blot, Immunofluorescence and Methyl DNA Immunoprecipitation, it has been shown to react with Human samples, but the sequence is not species specific so it should react with a wide range of sample types.
- 纯化方法
- Protein A Chromatography
- 免疫原
- This 5-Methylcytosine (5-mC) antibody was raised against 5-Methyl-cytidine conjugated to KLH and recognizes 5-Methylcytosine.
- 克隆位点
- A1
- 亚型
- IgG1
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anti-5-Methylcytosine antibody
MeDIP, DB, ICC, ELISA, IHC (p) 宿主: 兔 Monoclonal RM231 unconjugated
anti-5-Methylcytosine antibody (Biotin)IF, ICC 宿主: 小鼠 Monoclonal 5MC-CD Biotin
anti-5-Methylcytosine antibody (FITC)IF, ICC 宿主: 小鼠 Monoclonal 5MC-CD FITC
anti-5-Methylcytosine antibodyIF, ICC 宿主: 小鼠 Monoclonal 5MC-CD unconjugated
anti-5-Methylcytosine antibodyWB, FACS, IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-5-Methylcytosine antibodyIF, ICC 宿主: 小鼠 Monoclonal 5MC-CD unconjugated
anti-5-Methylcytosine antibodyMeDIP, FACS, IF, DB, IP, ELISA 宿主: 小鼠 Monoclonal b unconjugated
anti-5-Methylcytosine antibodyWB, MeDIP, FACS, IF, DB, ICC, ELISA, IHC 宿主: 小鼠 Monoclonal 33D3 unconjugated
anti-5-Methylcytosine antibodyMeDIP, DB, MeDIP-Seq 宿主: 小鼠 Monoclonal A1 unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 缓冲液
- Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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- 抗原
- 5-Methylcytosine
- 别名
- 5-Methylcytosine (5-mC)
- 物质类
- Chemical
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