5-Methylcytosine (5MeC) 抗体
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北京 101111
Quick Overview for 5-Methylcytosine (5MeC) 抗体 (ABIN2668982)
抗原
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- 5-Methylcytosine (5-mC) antibody (mAb) (Clone 33D3)
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纯化方法
- Protein A Chromatography
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免疫原
- Clone 33D3 recognizes the modified base 5-methylcytidine found in plant and vertebrate DNA.
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亚型
- IgG
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anti-5-Methylcytosine (5MeC) antibody
5MeC WB, FACS, IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-5-Methylcytosine (5MeC) antibody5MeC 适用: 人 IF, MeDIP, DB 宿主: 小鼠 Monoclonal A1 unconjugated
anti-5-Methylcytosine (5MeC) antibody (FITC)5MeC IF, ICC 宿主: 小鼠 Monoclonal 5MC-CD FITC
anti-5-Methylcytosine (5MeC) antibody5MeC IF, ICC 宿主: 小鼠 Monoclonal 5MC-CD unconjugated
anti-5-Methylcytosine (5MeC) antibody (Biotin)5MeC IF, ICC 宿主: 小鼠 Monoclonal 5MC-CD Biotin
anti-5-Methylcytosine (5MeC) antibody5MeC IF, MeDIP, DB 宿主: 小鼠 Monoclonal A1 unconjugated
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应用备注
- Validated Applications: MeDIP: 1 μg per IP IHC (FFPE): 1:1000 ELISA: 1:10,000 dilution Published Applications: MeDIP ICC/IF Flow Cytometry IHC DB
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限制
- 仅限研究用
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浓度
- 1 μg/μL
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缓冲液
- Purified IgG in PBS ( pH 7.4).
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- -20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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: "DNA Hypomethylation and Histone Variant macroH2A1 Synergistically Attenuate Chemotherapy-Induced Senescence to Promote Hepatocellular Carcinoma Progression." in: Cancer research, Vol. 76, Issue 3, pp. 594-606, (2016) (PubMed).
: "Paternal under-nutrition programs metabolic syndrome in offspring which can be reversed by antioxidant/vitamin food fortification in fathers." in: Scientific reports, Vol. 6, pp. 27010, (2016) (PubMed).
: "PTP1B controls non-mitochondrial oxygen consumption by regulating RNF213 to promote tumour survival during hypoxia." in: Nature cell biology, Vol. 18, Issue 7, pp. 803-813, (2016) (PubMed).
: "Hemi-methylated DNA regulates DNA methylation inheritance through allosteric activation of H3 ubiquitylation by UHRF1." in: eLife, Vol. 5, (2016) (PubMed).
: "Iodine-125 irradiation inhibits invasion of gastric cancer cells by reactivating microRNA-181c expression." in: Oncology letters, Vol. 12, Issue 4, pp. 2789-2795, (2016) (PubMed).
: "Ontogeny, distribution and potential roles of 5-hydroxymethylcytosine in human liver function." in: Genome biology, Vol. 14, Issue 8, pp. R83, (2014) (PubMed).
: "Stress-mediated p38 activation promotes somatic cell reprogramming." in: Cell research, Vol. 23, Issue 1, pp. 131-41, (2013) (PubMed).
: "Alteration of genic 5-hydroxymethylcytosine patterning in olfactory neurons correlates with changes in gene expression and cell identity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 36, pp. 14682-7, (2013) (PubMed).
: "Hydroxylation of 5-methylcytosine by TET2 maintains the active state of the mammalian HOXA cluster." in: Nature communications, Vol. 3, pp. 818, (2012) (PubMed).
: "DNA global hypomethylation in EBV-transformed interphase nuclei." in: Experimental cell research, Vol. 249, Issue 1, pp. 46-53, (1999) (PubMed).
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- 5-Methylcytosine (5MeC)
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别名
- 5-Methylcytosine
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物质类
- Chemical
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背景
- 5-Methylcytosine (5-Methylcytidine) is a modified base that is found in the DNA of plants and vertebrates. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. The 5-methylcytidine antibody (Clone 33D3) has been developed to discriminate between the modified base and its normal cytosine counterpart, allowing for gene promoter methylation analysis.
抗原
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