BRAF 抗体 (AA 424-453)
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Quick Overview for BRAF 抗体 (AA 424-453) (ABIN3029542)
抗原
See all BRAF 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 424-453
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交叉反应 (详细)
- Expected species reactivity: Mouse, Chicken
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纯化方法
- Antigen affinity purified
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免疫原
- A portion of amino acids 424-453 from the human protein was used as the immunogen for this BRAF antibody.
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亚型
- Ig Fraction
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应用备注
- The stated application concentrations are suggested starting amounts.Titration of the BRAF antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- In 1X PBS, pH 7.4, with 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at 4°C for up to one month. For long term, aliquot the BRAF antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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- BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))
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别名
- BRAF
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背景
- BRAF is a protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway. [UniProt]
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UniProt
- P15056
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途径
- MAPK Pathway, RTK signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy
抗原
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