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c-MET 抗体 (Activation Domain)

Name: c-MET 抗体 (Activation Domain)
SKU: ABIN492622
Availability: OutOfStock

This 小鼠单克隆 antibody specifically detects c-MET in WB. It exhibits reactivity toward 人.

Western Blotting (WB) image for anti-Met Proto-Oncogene (MET) (Activation Domain) antibody (ABIN492622) (c-MET 抗体 (Activation Domain))

1 image

产品编号 ABIN492622

发货至: 中国

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中国
北京 101111

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Beijing Economic Technological Development Area

Room 801-803

4A Biotech Co.,Ltd.

Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for c-MET 抗体 (Activation Domain) (ABIN492622)

抗原

See all c-MET (MET) 抗体

c-MET (MET) (Met Proto-Oncogene (MET))

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This c-MET antibody is un-conjugated

应用范围

Western Blotting (WB)

克隆位点

14G9

Discover our top product MET Primary Antibody

抗原表位
50

42

36

28

13

12

9

7

7

7

5

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2

2

2

2

2

2

2

1

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1

1

1

1

1

1

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1
Activation Domain

特异性

This monoclonal MET antibody (14G9) recognizes Met activation loop.

产品特性

Synonyms: Hepatocyte growth factor receptor, MET, Scatter factor receptor, HGF/SF receptor, c-Met,Met proto-oncogene tyrosine kinase

纯化方法

Subsequent Ultrafiltration and Size Exclusion Chromatography.

组件

incl. positive Control

免疫原

peptide conjugated to hemocyaninEpitope: kinase activation loop.

亚型

IgG1
anti-Met Proto-Oncogene (MET) (AA 25-150) antibody
MET 适用: 人, 小鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Met Proto-Oncogene (MET) (AA 743-932) antibody
MET 适用: 人, 猴 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 5E9C4 unconjugated

anti-Met Proto-Oncogene (MET) (AA 1092-1379) antibody
MET 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Met Proto-Oncogene (MET) antibody
MET 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Monoclonal 2D12 unconjugated Recombinant Antibody

anti-Met Proto-Oncogene (MET) (C-Term) antibody
MET 适用: 人 WB, IF, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Met Proto-Oncogene (MET) (AA 743-932) antibody
MET 适用: 人 ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 5E9B7 unconjugated

anti-Met Proto-Oncogene (MET) (Internal Region) antibody
MET 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Met Proto-Oncogene (MET) (Tyr1003) antibody
MET 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Met Proto-Oncogene (MET) (AA 83-209) antibody
MET 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Met Proto-Oncogene (MET) (pTyr1234) antibody
MET 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated A431 cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

实验流程

Positive Control Provided. Cell lysate from untreated A431 cellsDescription: Cell lysate from untreated A431 cells, epidermoid carcinoma (Human)Format: Lyophilized cell lysate from serum starved A431 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.

限制

仅限研究用
溶解方式

Restore with 1 mL H2O (15 min, RT).

缓冲液

1 mLPBS, containing 0.09 % Sodium Azide/PEG and Sucrose.

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freeze / thaw cycles. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.

有效期

12 months
抗原

c-MET (MET) (Met Proto-Oncogene (MET))

别名

HGF Receptor

背景

MET is a high affinity receptor for Hepatocyte Growth Factor/Scatter Factor HGF/SF. The (HGF/SF)-(HGFR/MET) ligand-receptor system appears to have an important role in controlling interaction between mesenchymal cells and other cell types and has been implicated in the cell mitogenic response, cell motility and development. The interaction with HGF leads to autophosphorylation at multiple tyrosine residues and recruiting of several downstream signaling components (Gab1, c-cbl, PI3-kinase). The phosphorylation of Tyr 1234/1235 leads to kinase activation, and phospho-Tyr 1349 is the Gab1 binding site. Altered MET levels and/or tyrosine kinase activities have been found in different tumors, such as colon, renal, and breast cancer.Synonyms: HGF/SF receptor, Hepatocyte growth factor receptor, MET, Met proto-oncogene tyrosine kinase, Scatter factor receptor, c-Met

基因ID

4233

UniProt

P08581

途径

RTK signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor