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- 抗原 See all TNFRSF8 抗体
- TNFRSF8 (Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This TNFRSF8 antibody is conjugated to FITC
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应用范围
- Flow Cytometry (FACS)
- 特异性
- This antibody reacts with CD30 on Flow Cytometry.
- 交叉反应 （详细）
- Species reactivity (tested):Human.
- 产品特性
- Synonyms: TNFRSF8, D1S166E, CD30L receptor, KI-1 antigen, Tumor necrosis factor receptorsuperfamily member 8, Lymphocyte activation antigen CD30
- 纯化方法
- Protein-A Agarose Chromatography of hybridoma supernatant.
- 免疫原
- Co cell line cellsRemarks: Hybridoma was established by fusion of mouse myeloma cell with Balb/c mousesplenocyte
- 克隆位点
- Ber-H2
- 亚型
- IgG1
- Top Product
- Discover our top product TNFRSF8 Primary Antibody
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anti-Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) (AA 19-379) antibody
TNFRSF8 适用: 人 IHC, WB, IHC (p) 宿主: 小鼠 Monoclonal 18D3 unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) antibodyTNFRSF8 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 3B10 unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) antibodyTNFRSF8 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) (AA 19-379) antibodyTNFRSF8 适用: 人 FACS, IHC, WB, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 4D1C2 unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) (AA 131-230) antibodyTNFRSF8 适用: 人, Pig FACS, WB, IHC (p), ELISA, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) antibodyTNFRSF8 适用: 人 FACS, IHC (p) 宿主: 小鼠 Monoclonal MEM-268 unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) antibody (APC)TNFRSF8 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-268 APC
anti-Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) antibodyTNFRSF8 适用: 人 IF, WB 宿主: 兔 Monoclonal unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) antibodyTNFRSF8 适用: 人 FACS, WB, IHC (p), IP 宿主: 小鼠 Monoclonal Ber-H2 unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) (AA 51-150) antibodyTNFRSF8 适用: 大鼠 IHC (p), ELISA, IF (p), IF (cc), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Flow Cytometry: 5-10 μg/mL (final concentration). Detailed procedure is provided in Protocols.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Flow cytometric analysis for floating cellsWe usually use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN3]. 2) Resuspend the cells with washing buffer (5x10e6 cells/mL). 3) Add 50 µL of the cell suspension into each tube, and centrifuge at 500 x g for 1 minute atRT (20~25°C). Remove supernatant by careful aspiration. 4) Add 10 µL of normal goat serum containing 1 mg/mL normal human IgG and 0. 1% NaN3or 20 µL of Clear Back (human Fc receptor blocking reagent) to the cell pellet after tapping. Mix well and incubate for 5 minutes at room temperature. 5) Add 20 µL of the FITC labeled CD30 monoclonal antibody (Ber-H2) (5-10 µg/mL) dilutedwith the washing buffer. Mix well and incubate for 30 minutes at RT (20~25°C). 6) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at RT. Remove supernatant by careful aspiration. 7) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Positive Control: CCRF-CEMFlow cytometric analysis for whole blood cellsWe usually use Falcon tubes or equivalents as reaction tubes for all steps described below. 1) Add 20 µL of the FITC labeled CD163 monoclonal antibody (Ber-Mac3) (5-10 µg/mL)diluted with the washing buffer [PBS containing 2% fetal calf serum(FCS) and 0. 1% NaN3] into each tube. 2) Add 50 µL of whole blood into each tube. Mix well, and incubate for 30 minutes at RT(20~25°C). 3) Lyse with OptiLyse C (for analysis on Beckman Coulter instruments) or OptiLyse B (foranalysis on BD instruments), using the procedure recommended in the respective packageinserts. 4) Add 1mL of H2O to each tube and incubate for 10 minutes at RT (20~25°). 5) Centrifuge at 500 x g for 1 minute at RT (20~25°C). Remove supernatant by carefulaspiration. 6) Add 1 mL of washing buffer followed by centrifugation at 500 x g for 1 minute at RT(20~25°C). Remove supernatant by careful aspiration. 7) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer.
- 限制
- 仅限研究用
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- 浓度
- 50 µg/mL
- 缓冲液
- PBS containing 5 % BSA and 0.09 % Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
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Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- TNFRSF8 (Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))
- 别名
- CD30 (TNFRSF8 产品)
- 别名
- CD30 antibody, D1S166E antibody, Ki-1 antibody, Cd30 antibody, Ki antibody, TNFRSF8 antibody, TNF receptor superfamily member 8 antibody, tumor necrosis factor receptor superfamily, member 8 antibody, TNFRSF8 antibody, Tnfrsf8 antibody
- 背景
- CD30, also known as Ki-1, TNFRSF8, or Be-H2, is a 120 kDa glycoprotein expressed on the surface of mitogen-activated B-cells and T-cells but not on resting lymphocytes or monocytes. CD30 is also a marker for Hodgkin and Sternberg-Reed cells of Hodgkin's lymphomas and related hematologic malignancies. Soluble forms of CD30 have been found in the serum of patients with adult T-cell leukemia or other CD30+ lymphomas. The CD30 ligand, CD153, is a type II transmembrane glycoprotein that enhances proliferation of activated T-cells and induces apoptosis in CD30+ lymphoma-derived cell lines.Synonyms: CD30L receptor, D1S166E, KI-1 antigen, Lymphocyte activation antigen CD30, TNFRSF8, Tumor necrosis factor receptor superfamily member 8
- 基因ID
- 943
- UniProt
- P28908
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