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TLR4 抗体 (N-Term)

Name: TLR4 抗体 (N-Term)
SKU: ABIN4886746
Availability: OutOfStock

TLR4 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

Anti-TLR4 antibody, Western blotting Lane 1: HELA Cell Lysate Lane 2: SMMC Cell Lysate (TLR4 抗体 (N-Term))

Anti-TLR4 antibody, IHC(P): Human Lung Cancer Tissue (TLR4 抗体 (N-Term))

IHC

2 images

(29 references)

产品编号 ABIN4886746

发货至: 中国

Datasheet as PDF

抗原 See all TLR4 抗体

TLR4 (Toll-Like Receptor 4 (TLR4))
抗原表位
16

16

16

16

8

8

7

4

4

4

4

3

3

3

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
AA 50-68, N-Term
适用
178

93

75

37

23

20

19

19

5

2

1

1
人
宿主
134

70

5

4
兔
Compatible Secondaries
克隆类型
138

75
多克隆
标记
97

22

20

14

6

6

5

3

3

3

3

3

3

3

3

3

3

2

1

1

1

1

1

1

1

1

1

1

1
This TLR4 antibody is un-conjugated
应用范围
155

126

56

50

35

29

27

27

26

24

13

8

8

7

5

5

4

2

1

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

原理

Rabbit IgG polyclonal antibody for Toll-like receptor 4(TLR4) detection. Tested with WB, IHC-P in Human.

序列

DNLPFSTKNL DLSFNPLRH

交叉反应（详细）

No cross reactivity with other proteins.

产品特性

Rabbit IgG polyclonal antibody for Toll-like receptor 4(TLR4) detection. Tested with WB, IHC-P in Human.
Gene Name: toll-like receptor 4
Protein Name: Toll-like receptor 4

纯化方法

Immunogen affinity purified.

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human TLR4(50-68aa DNLPFSTKNLDLSFNPLRH).

亚型

IgG

Top Product

Discover our top product TLR4 Primary Antibody
anti-Toll-Like Receptor 4 (TLR4) (AA 751-835) antibody
TLR4 适用: 人, 小鼠, 大鼠, Cow WB, FACS, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody
TLR4 适用: 人 WB, FACS, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Toll-Like Receptor 4 (TLR4) (AA 49-247) antibody
TLR4 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (HRP)
TLR4 适用: 人 WB, FACS, IHC, IF, ICC 宿主: 兔 Polyclonal HRP

anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (FITC)
TLR4 适用: 人 WB, FACS, IHC, IF, ICC 宿主: 兔 Polyclonal FITC

anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (Alkaline Phosphatase (AP))
TLR4 适用: 人 WB, FACS, IHC, IF, ICC 宿主: 兔 Polyclonal Alkaline Phosphatase (AP)

anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (Biotin)
TLR4 适用: 人 WB, FACS, IHC, IF, ICC 宿主: 兔 Polyclonal Biotin

anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (PE)
TLR4 适用: 人 WB, FACS, IHC, IF, ICC 宿主: 兔 Polyclonal PE

anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (APC)
TLR4 适用: 人 WB, FACS, IHC, IF, ICC 宿主: 兔 Polyclonal APC

anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (PerCP)
TLR4 适用: 人 WB, FACS, IHC, IF, ICC 宿主: 兔 Polyclonal PerCP

应用备注

WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

储存液

Sodium azide, Thimerosal (Merthiolate)

注意事项

This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C/-20 °C

储存方法

At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Sun, Li, Ma, Feng, Song, Lv, He: "Inhibition of HMGB1 reduces rat spinal cord astrocytic swelling and AQP4 expression after oxygen-glucose deprivation and reoxygenation via TLR4 and NF-κB signaling in an IL-6-dependent manner." in: Journal of neuroinflammation, Vol. 14, Issue 1, pp. 231, (2018) (PubMed).

Zhang, Fu, Tang: "The protective effects of magnolol on acute trinitrobenzene sulfonic acid‑induced colitis in rats." in: Molecular medicine reports, Vol. 17, Issue 3, pp. 3455-3464, (2018) (PubMed).

Sun, Xiu, Wang, Brigstock, Li, Qu, Gao: "Lipopolysaccharide enhances TGF-β1 signalling pathway and rat pancreatic fibrosis." in: Journal of cellular and molecular medicine, Vol. 22, Issue 4, pp. 2346-2356, (2018) (PubMed).

Sun, Xue, Xue, Ren, Wu, Wang: "Acetylpuerarin protects against OGD-induced cell injury in BV2 microglia by inhibiting HMGB1 release." in: Die Pharmazie, Vol. 73, Issue 2, pp. 92-97, (2018) (PubMed).

Gong, Guan, Tian, Li, Zhang: "Rho Kinase Type 1 (ROCK1) Promotes Lipopolysaccharide-induced Inflammation in Corneal Epithelial Cells by Activating Toll-Like Receptor 4 (TLR4)-Mediated Signaling." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 24, pp. 3514-3523, (2018) (PubMed).

Jin, Zhang, Zhao, Xu, Shao, Huang, Shi, Yang: "Responses of Intestinal Mucosal Barrier Functions of Rats to Simulated Weightlessness." in: Frontiers in physiology, Vol. 9, pp. 729, (2018) (PubMed).

Liu, Han, Chen, Huang, Zhao, Berta, Gao, Ji: "Toll-like receptor 4 contributes to chronic itch, alloknesis, and spinal astrocyte activation in male mice." in: Pain, Vol. 157, Issue 4, pp. 806-17, (2017) (PubMed).

Xu, Xue, Zhang, Qu: "Knockdown of versican V1 induces a severe inflammatory response in LPS-induced acute lung injury via the TLR2-NF-κB signaling pathway in C57BL/6J mice." in: Molecular medicine reports, Vol. 13, Issue 6, pp. 5005-12, (2017) (PubMed).

Zhou, He, Wang, Xie, Liu, Liu, Song, Ma: "Intravenous Administration Is an Effective and Safe Route for Cancer Gene Therapy Using the Bifidobacterium-Mediated Recombinant HSV-1 Thymidine Kinase and Ganciclovir." in: International journal of molecular sciences, Vol. 17, Issue 6, (2017) (PubMed).

Shanshan, Beibei, Li, Minna, Shipeng, Li, Yong: "Phospholipase A2 of Peroxiredoxin 6 Plays a Critical Role in Cerebral Ischemia/Reperfusion Inflammatory Injury." in: Frontiers in cellular neuroscience, Vol. 11, pp. 99, (2017) (PubMed).

Wang, Jiao, Zhao, Wang, Sun, Lin: "Allicin Alleviates Reticuloendotheliosis Virus-Induced Immunosuppression via ERK/Mitogen-Activated Protein Kinase Pathway in Specific Pathogen-Free Chickens." in: Frontiers in immunology, Vol. 8, pp. 1856, (2017) (PubMed).

Ansari, Li, Sun, Huang, Zhao, Cui, Hu, Zhong, Karrow, Liu: "Lipopolysaccharide induces acute bursal atrophy in broiler chicks by activating TLR4-MAPK-NF-κB/AP-1 signaling." in: Oncotarget, Vol. 8, Issue 65, pp. 108375-108391, (2017) (PubMed).

Huang, Xiao, Lu, Yang, Ansari, Khaliq, Song, Zhong, Liu, Peng: "Increased Thymic Cell Turnover under Boron Stress May Bypass TLR3/4 Pathway in African Ostrich." in: PLoS ONE, Vol. 10, Issue 6, pp. e0129596, (2016) (PubMed).

Cui, Feng, Zhang, Jia: "The anti-inflammation effect of baicalin on experimental colitis through inhibiting TLR4/NF-?B pathway activation." in: International immunopharmacology, Vol. 23, Issue 1, pp. 294-303, (2015) (PubMed).

Xu, Wang, Wu, Wang: "Pyrrolidine Dithiocarbamate Inhibits Nuclear Factor ?B and Toll-Like Receptor 4 Expression in Rats with Acute Necrotizing Pancreatitis." in: Gut and liver, Vol. 9, Issue 3, pp. 411-6, (2015) (PubMed).

Liu, Hu, Lian, Liu, Liu, Liu, Lin, Liu, Duan, Yong, Ye: "Dual-color immunofluorescent labeling with quantum dots of the diabetes-associated proteins aldose reductase and Toll-like receptor 4 in the kidneys of diabetic rats." in: International journal of nanomedicine, Vol. 10, pp. 3651-62, (2015) (PubMed).

Wang, Wang, Fang, Zhou, Liu, Su: "High glucose induces and activates Toll-like receptor 4 in endothelial cells of diabetic retinopathy." in: Diabetology & metabolic syndrome, Vol. 7, pp. 89, (2015) (PubMed).

Li, Hu, Yang, Zhang, Waqas, Liu, Zhang, Wang, Chen, Le, Ullah, Chen: "Expression of TLR2/4 in the sperm-storing oviduct of the Chinese soft-shelled turtle Pelodiscus sinensis during hibernation season." in: Ecology and evolution, Vol. 5, Issue 19, pp. 4466-79, (2015) (PubMed).

Li, Qian, Ju, Wang: "Upregulation of Toll-like receptor 2 expression in colorectal cancer infected by human cytomegalovirus." in: Oncology letters, Vol. 9, Issue 1, pp. 365-370, (2014) (PubMed).

Yuan, Zhang, Yang: "Ligusticum wallichii Extract Inhibited the Expression of IL-1? after AMI in Rats." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2014, pp. 620359, (2014) (PubMed).

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抗原

TLR4 (Toll-Like Receptor 4 (TLR4))

别名

TLR4 (TLR4 产品)

别名

ARMD10 antibody, CD284 antibody, TLR-4 antibody, TOLL antibody, Lps antibody, Ly87 antibody, Ran/M1 antibody, Rasl2-8 antibody, toll like receptor 4 antibody, toll-like receptor 4 antibody, TLR4 antibody, Tlr4 antibody

背景

TLR4, Toll-like receptor 4, is a protein that in humans is encoded by the TLR4 gene. TLR 4 is a toll-like receptor. TLR4, the human homolog of Drosophila Toll, is a type I transmembrane protein with an extracellular domain consisting of a leucine-rich repeat region and an intracellular domain homologous to that of human interleukin-1 receptor. The TLR4 gene is mapped to chromosome 9q32-q33 by fluorescence in situ hybridization.It detects lipopolysaccharide from Gram-negative bacteria and is thus important in the activation of the innate immune system.The protein encoded by this gene is a member of the Toll-like receptor(TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns(PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity.

Synonyms: ARMD10 antibody|CD284 antibody|CD284 antigen antibody|Homolog of Drosophila toll antibody|hToll antibody|TLR 4 antibody|TLR4 antibody|TLR4_HUMAN antibody|Toll(Drosophila) homolog antibody|TOLL antibody|Toll Endotoxin Hyporesponsiveness antibody|Toll like receptor 4 antibody|Toll like receptor 4 precursor antibody|Toll-like receptor 4 antibody

UniProt

O00206

途径

TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins