Caspase 8 抗体 (N-Term)
(9 references)-
- 抗原 See all Caspase 8 (CASP8) 抗体
- Caspase 8 (CASP8)
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抗原表位
- AA 410-449, N-Term
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Caspase 8 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 原理
- Anti-Caspase 8/CASP8 Antibody Picoband®
- 序列
- VSYRNPAEGT WYIQSLCQSL RERCPRGDDI LTILTEVNYE
- 交叉反应 （详细）
- No cross-reactivity with other proteins.
- 产品特性
- Anti-Caspase 8/CASP8 Antibody ((ABIN4886501)). Tested in Flow Cytometry, IHC, ICC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human Caspase 8, different from the related mouse and rat sequences by seven amino acids.
- 亚型
- IgG
- Top Product
- Discover our top product CASP8 Primary Antibody
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anti-Caspase 8 (CASP8) (AA 411-482) antibody
CASP8 适用: 人, 小鼠, 大鼠, 猴, Pig, 绵羊 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Caspase 8 (CASP8) (AA 1-479) antibodyCASP8 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Caspase 8 (CASP8) (AA 188-280) antibodyCASP8 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Caspase 8 (CASP8) (AA 217-384) antibodyCASP8 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Caspase 8 (CASP8) (AA 385-479) antibodyCASP8 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Caspase 8 (CASP8) (AA 7-201) antibodyCASP8 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Caspase 8 (CASP8) antibodyCASP8 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 1H11 unconjugated
anti-Caspase 8 (CASP8) (AA 217-374) antibodyCASP8 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Caspase 8 (CASP8) (pSer347) antibodyCASP8 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Caspase 8 (CASP8) antibodyCASP8 适用: 小鼠 WB, IHC, ELISA, FACS 宿主: 大鼠 Monoclonal 1G12 unconjugated
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- 应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry, 0.5-1 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Activated neutrophils induce epithelial cell apoptosis through oxidant-dependent tyrosine dephosphorylation of caspase-8.Jia SH, et al. Am J Pathol, 2014 Apr. 2. Association between CASP8 -652 6N del polymorphism (rs3834129) and colorectal cancer risk: results from a multi-centric study.Pardini B, et al. PLoS One, 2014. 3. Intra- and interdimeric caspase-8 self-cleavage controls strength and timing of CD95-induced apoptosis.Kallenberger SM, et al. Sci Signal, 2014 Mar 11. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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: "Di-2-pyridylhydrazone Dithiocarbamate Butyric Acid Ester Exerted Its Proliferative Inhibition against Gastric Cell via ROS-Mediated Apoptosis and Autophagy." in: Oxidative medicine and cellular longevity, Vol. 2018, pp. 4950705, (2018) (PubMed).
: "[Corrigendum] Inhibitory activity of apogossypol in human prostate cancer in vitro and in vivo." in: Molecular medicine reports, Vol. 17, Issue 6, pp. 8010, (2018) (PubMed).
: "Intravenous Administration Is an Effective and Safe Route for Cancer Gene Therapy Using the Bifidobacterium-Mediated Recombinant HSV-1 Thymidine Kinase and Ganciclovir." in: International journal of molecular sciences, Vol. 17, Issue 6, (2017) (PubMed).
: "Exopolysaccharides from Lactobacillus plantarum NCU116 induce c-Jun dependent Fas/Fasl-mediated apoptosis via TLR2 in mouse intestinal epithelial cancer cells." in: Scientific reports, Vol. 7, Issue 1, pp. 14247, (2017) (PubMed).
: "2-DG-Regulated RIP and c-FLIP Effect on Liver Cancer Cell Apoptosis Induced by TRAIL." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 21, pp. 3442-8, (2016) (PubMed).
: "Croton Tiglium Extract Induces Apoptosis via Bax/Bcl-2 Pathways in Human Lung Cancer A549 Cells" in: Asian Pacific journal of cancer prevention : APJCP, Vol. 17, Issue 11, pp. 4893-4898, (2016) (PubMed).
: "Inhibitory activity of apogossypol in human prostate cancer in vitro and in vivo." in: Molecular medicine reports, Vol. 11, Issue 6, pp. 4142-8, (2015) (PubMed).
: "Sorafenib sensitizes (-)-gossypol-induced growth suppression in androgen-independent prostate cancer cells via Mcl-1 inhibition and Bak activation." in: Molecular cancer therapeutics, Vol. 11, Issue 2, pp. 416-26, (2012) (PubMed).
: "[Relation between the sensitivity of the taste analyzer and high blood pressure]." in: Vnitr̆ní lékar̆ství, Vol. 32, Issue 7, pp. 642-6, (1986) (PubMed).
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: "Di-2-pyridylhydrazone Dithiocarbamate Butyric Acid Ester Exerted Its Proliferative Inhibition against Gastric Cell via ROS-Mediated Apoptosis and Autophagy." in: Oxidative medicine and cellular longevity, Vol. 2018, pp. 4950705, (2018) (PubMed).
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- 抗原
- Caspase 8 (CASP8)
- 别名
- CASP8 (CASP8 产品)
- 背景
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Synonyms: CAP4, MACH, MCH5, FLICE, ALPS2B, Casp-8, Caspase-8, Apoptotic cysteine protease, CASP-8
Tissue Specificity: Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.
Background: CASP8 is also known as CAP4, MACH or MCH5. This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. In addtion, this protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.
- 分子量
- 55 kDa
- 基因ID
- 841
- UniProt
- Q14790
- 途径
- Apoptosis, Caspase Cascade in Apoptosis, TLR signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades
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