KDELR1 抗体 (C-Term)
KDELR1
适用: Drosophila melanogaster, 植物, Saccharomyces cerevisiae
WB, IF
宿主: 小鼠
Monoclonal
2-00E-007
unconjugated
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- 抗原 See all KDELR1 抗体
- KDELR1 (KDEL (Lys-Asp-Glu-Leu) Endoplasmic Reticulum Protein Retention Receptor 1 (KDELR1))
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抗原表位
- C-Term
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适用
- Drosophila melanogaster, 植物, Saccharomyces cerevisiae
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This KDELR1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF)
- 特异性
- Detects ~78 kDa protein. The 2E7 clone recognizes the C-terminal peptide HDEL, a common version of the endoplasmic reticulum retention signal found in Yeast, Plant, nematode and other ER proteins. It specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum and wheat (4).
- 交叉反应 （详细）
- Species reactivity (tested):Drosophila, Yeast, Saccharomyce, Plants (barnyard grass, beet, cotton, mung bean, sorghum and wheat).
- 产品特性
- Synonyms: KDEL endoplasmic reticulum protein retention receptor 1, ERD2.1, ER marker, ER lumenprotein retaining receptor 1, Putative MAPK-activating protein PM23
- 纯化方法
- Protein G Chromatography.
- 免疫原
- A synthetic HDEL peptide corresponding to the C-terminus of Yeast Bip.
- 克隆位点
- 2-00E-007
- 亚型
- IgG2b
- Top Product
- Discover our top product KDELR1 Primary Antibody
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anti-KDEL (Lys-Asp-Glu-Leu) Endoplasmic Reticulum Protein Retention Receptor 1 (KDELR1) (Internal Region) antibody
KDELR1 适用: 人, 小鼠, 大鼠 WB, IF, ICC, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-KDEL (Lys-Asp-Glu-Leu) Endoplasmic Reticulum Protein Retention Receptor 1 (KDELR1) (AA 192-212) antibodyKDELR1 适用: 人, Cow, 小鼠, 大鼠, 猴, 犬 WB, IP, ICC, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western blot: 1/1000-1/2000.1 μg/mL of SMC-175 was sufficient for detection of HDEL-containing proteins in 10 μg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG -HRP asthe secondary antibody. Immunofluorescence: 1/50-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH 7.4 containing 50 % Glycerol with 0.09 % Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- KDELR1 (KDEL (Lys-Asp-Glu-Leu) Endoplasmic Reticulum Protein Retention Receptor 1 (KDELR1))
- 别名
- KDEL Receptor 1 / KDELR1 (KDELR1 产品)
- 别名
- ERD2 antibody, ERD2.1 antibody, HDEL antibody, PM23 antibody, 8030486F04Rik antibody, AW215843 antibody, Erd2.1 antibody, KDEL endoplasmic reticulum protein retention receptor 1 antibody, KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1 antibody, KDELR1 antibody, Kdelr1 antibody
- 背景
- HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones, facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus, and aid in the proper folding of nascent polypeptide chains (1, 2). GRP 78 is localized in the endoplasmic reticulum (ER), where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains (2). Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually KDEL in animal cells, and HDEL in S.cerevisiae (3).Synonyms: ER lumen protein retaining receptor 1, ER marker, ERD2.1, KDEL endoplasmic reticulum protein retention receptor 1, Putative MAPK-activating protein PM23
- 基因ID
- 10945
- UniProt
- P24390
- 途径
- Maintenance of Protein Location
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