CHEK2 抗体
Quick Overview for CHEK2 抗体 (ABIN487312)
抗原
See all CHEK2 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody reacts with Human Chk2.
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交叉反应 （详细）
- Species reactivity (tested):Human.
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产品特性
- Synonyms: CHEK2, CHEK-2, CHK-2, RAD53, Cds1, Serine/threonine-protein kinase Chk2, CHK2checkpoint homolog
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纯化方法
- Protein-A Sepharose Chromatography.
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免疫原
- Full-length Human Chk2 fusion protein. Remarks: Hybridoma was established by fusion of Mouse myeloma cell NS-2 with Balb/cmouse splenocyte
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亚型
- IgG2a
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anti-Checkpoint Kinase 2 (CHEK2) (pThr387) antibody
CHEK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Checkpoint Kinase 2 (CHEK2) (AA 1-543) antibodyCHEK2 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Checkpoint Kinase 2 (CHEK2) (AA 1-543) antibodyCHEK2 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 4B7 unconjugated
anti-Checkpoint Kinase 2 (CHEK2) (pSer516) antibodyCHEK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Checkpoint Kinase 2 (CHEK2) (pThr68) antibodyCHEK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Checkpoint Kinase 2 (CHEK2) (pThr383) antibodyCHEK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Checkpoint Kinase 2 (CHEK2) antibodyCHEK2 适用: 人, 犬 WB, IHC, IP, IHC (p), FACS 宿主: 小鼠 Monoclonal 2H5 unconjugated
anti-Checkpoint Kinase 2 (CHEK2) antibodyCHEK2 适用: 人, 犬 WB, IHC, IF, IP, IHC (p), FACS 宿主: 小鼠 Monoclonal 5C4 unconjugated
anti-Checkpoint Kinase 2 (CHEK2) antibodyCHEK2 适用: 人, 猴, 犬 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 10G7 unconjugated
anti-Checkpoint Kinase 2 (CHEK2) (AA 1-220) antibodyCHEK2 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: 0.2 μg/mL. Positive Control: HeLa. Immunoprecipitation: 3 μg/200-300 μL of cell extract. Positive Control: HeLa. Immunohistochemistry: 1-5 μg/mLHeat treatment is necessary for Paraffin Embedded Sections. Microwave oven: 2 times for 10 minutes each in citrate buffer ( pH 6.5). Positive Control: Tonsil Tissue. Detailed procedure is provided in Protocols.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- SDS-PAGE & Western Blotting1) Wash the cells 3 times with PBS and suspend with 10 volume of cold Lysis buffer (50 mMTris-HCl, pH 7. 2, 250 mM NaCl, 0. 1% NP-40, 2 mM EDTA, 10% glycerol) containingappropriate protease inhibitors. Incubate it at 4°C with rotating for 30 minutes, thensonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4°C and transfer the supernatant toanother tube. Measure the protein concentration of the supernatant and add the Lysisbuffer to make 8 mg/mL solution. 3) Mix the sample with equal volume of Laemmli’s sample buffer. 4) Boil the samples for 2 minutes and centrifuge. Load 10 μL of the sample per lane in a 1mm thick SDS-polyacrylamide gel for electrophoresis. 5) Blot the protein to a polyvinylidene difluoride (PVDF) membrane at 1 mA/cm2 for 1 hourin a semi-dry transfer system. (Transfer Buffer: 25 mM Tris, 190 mM glycine, 20% MeOH). See the manufacture's manual for the transfer procedure. 6) To reduce nonspecific binding, soak the membrane in 10% skimmed milk (in PBS, pH7. 2) for 1 hour at room temperature, or overnight at 4°C. 7) Incubate the membrane with the anti-Chk2 (DCS-270) monoclonal antibody (0. 2 μg/mL)diluted with 1% skimmed milk (in PBS, pH 7. 2) for 1 hour at room temperature. 8) Wash the membrane with PBS (5 minutes x 6 times). 9) Incubate the membrane with the 1: 10000 POD-conjugated anti-mouse IgG diluted with1% skimmed milk (in PBS, pH 7. 2) for 1 hour at room temperature. 10) Wash the membrane with PBS (5 minutes x 6 times). 11) Wipe excess buffer from the membrane, then incubate it with appropriatechemiluminescence reagents for 1 minute. Remove extra reagent from the membrane bydabbing with a paper towel, and seal it in plastic wrap. 12) Expose to an X-ray film in a dark room for 5 minutes. Develop the film as usual. Theconditions for exposure and development may vary. Positive Controls for Western blotting: HeLaImmunoprecipitation1) Wash the cells 3 times with PBS and suspend with 10 volume of cold Lysis buffer (50 mMTris-HCl, pH 7. 2, 250 mM NaCl, 0. 1% NP-40, 2 mM EDTA, 10% glycerol) containingappropriate protease inhibitors. Incubate it at 4°C with rotating for 30 minutes, thensonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4°C and transfer the supernatant toanother tube. 3) Add 3 µg of the anti-Chk2 (DCS-270) monoclonalantibody into 250 µL of the supernatant. Mix well and incubate with gentle agitation for30-120 minutes at 4°C. Add 20 µL of 50% Protein A-agarose beads resuspended in the
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.2 containing 50 % Glycerol without preservatives.
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储存液
- Without preservative
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储存条件
- -20 °C
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储存方法
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Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. -
有效期
- 12 months
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- CHEK2 (Checkpoint Kinase 2 (CHEK2))
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别名
- CHK2
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背景
- Checkpoint kinase 2 (Chk2), also known as Cds1, is a 61 kDa nuclear protein that functions as a checkpoint kinase to regulate the cell cycle progression following DNA damage. Chk2 inhibits CDC2 by inactivating CDC25, the phosphatase that normally activates CDC2. Other targets for Chk2 include the tumor suppressors BRCA1 and p53, which it stabilizes by phosphorylation of Ser20. Chk2 is itself phosphorylated and activated by ATM following DNA damage. Defects in Chk2 contribute to the development of human cancers, and implicate Chk2 as a candidate tumor suppressor and an attractive target for drug discovery.Synonyms: CHEK-2, CHEK2, CHK-2, CHK2 checkpoint homolog, Cds1, RAD53, Serine/threonine-protein kinase Chk2
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基因ID
- 11200
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UniProt
- O96017
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途径
- p53 Pathway, Apoptosis, Cell Division Cycle
抗原
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